刚刚记录了siRNA作为dsRNA合成所须的模板:,输入到【Enter accession number(s), gi(s), or FASTA sequence(s)】一栏(图 4.2)。 图4.2 3、选择Database 在Database处选择【Genomic + transcript databases】,因为我们要看设计的siRNA能靶向多少转录本。然后选择对应种属的库【Mouse genomic plus transcript (Mouse...
注意NCBI-BLAST 上的sequence ID要和NCBI-Gene上的ID一致,不然siRNA序列的具体位置会有差异, BLAST 上的sequence ID为NM_003012.4,sbject为768-778. Gene上的sequence ID为NM_003012.5,sbject为770-790. 当然啦,合适的siRNA序列只是实验成功的一小步,一篇高质量...
注意NCBI-BLAST 上的sequence ID要和NCBI-Gene上的ID一致,不然siRNA序列的具体位置会有差异, BLAST 上的sequence ID为NM_003012.4,sbject为768-778. Gene上的sequence ID为NM_003012.5,sbject为770-790. 本站仅提供存储服务,所有内容均由用户发布,如发现有害或侵权内容,请点击举报。
2. Paste the sequence of your target gene. (Spaces and numbers will be ignored) 3. Enter the desired motif size (21 nt by default). 4. If you want to increase the siRNA's specificity by removing non-unique sequences, choose the database corresponding to the target gene. (optional) On...
siRNAdb:adatabaseofsiRNAsequences AlistairM.Chalk*,RichardE.Warfinge,PatrickGeorgii-Hemmingand ErikL.L.Sonnhammer CenterforGenomicsandBioinformatics,KarolinskaInstitutet,Berzeliusva¨g35,S-17177Stockholm,Sweden ReceivedAugust15,2004;RevisedandAcceptedOctober282004 ...
5. To avoid off target effect, choose miRNA SEED database corresponding to the target gene. (optional) siRNA Name: Sequence (max: 9600bp): Motif size:nt Restrict the search to the region from position:to siRNA target sequence GC% between:and ...
In this work, we presented a database of SARS-CoV-2 targets for small interference RNA (siRNA) based approaches, aiming to speed the design process by providing a broad set of possible targets and siRNA sequences. The siRNAs sequences are characterized and evaluated by more than 170 features...
The siRNA database provides a gene-centric view of siRNA experimental data, including siRNAs of known efficacy and siRNAs predicted to be of high efficacy by a combination of methods. Linked to these sequences is information such as siRNA thermodynamic properties and the potential for sequence-...
This tool generates template DNA oligonucleotide sequences from an input siRNA target sequence. Designs for both the "two-oligonucleotide" and "single-oligonucleotide" approach are displayed. These designs are generated using the loop, terminator and RNA polymerase promoter sequences described in the ki...
Truss, M. et al. HuSiDa–the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells. Nucleic Acids Res. 33, D108–D111 (2005). 除此之外,从一些商业来源也可以获得有效的siRNAs(比如Qiagen的HP validat...