One downfall of this kit is that you must be very careful to perform all of the reaction steps in the dark to ensure a good yield. This can become quite time consuming when you must label a large quantity of siRNA, since the labeling must be done in smaller aliquots. The smaller aliquo...
Consequently, a web-based server, GC Content Calculator (http://www.endmemo.com/bio/gc.php), was utilized to calculate the exact percentages of GC content in predicted siRNA and miRNA. 2.4. Calculation of heat capacity and concentration plot of RNA-duplex The collective heat capacity of RNA-...