图2. Each data point represents a minimum of 30 siRNAs to 10 gene targets, tested in triplicate: Each siRNA was individually transfected into HeLa cells at the indicated siRNA concentration. After transfection (48 hr), RNA was isolated using the MagMAX™-96 ...
4. For 4 consecutive wells of 24-well plate, add the transfection complexes which are prepared with same concentration of DNA (0.5 µg per well) and different 4 concentrations of siRNA ranging from final 10 nM to 1.25 nM. 5. Check silencing effect in the 4 wells 24~48 hours post tran...
knockdown levels despite differences in cell density, minor experimental inaccuracies, and other variations. Not also does this make it easy to optimize Lipofectamine RNAiMAX reagent for the lowest siRNA concentration but also reduces cellular stress and cytotoxicity in your experimental system ...
Accell siRNA retains functionality following storage at a variety of conditions. Accell siRNA targeting human GAPD and Accell Non-targeting control siRNA (NTC) were stored at 1 mM concentration in RNAse-free water (10 μM data not shown) 由于Accell siRNA最终需要混合到培养基中,实验结果表明,加入...
A method of measuring the concentration of an siRNA by single molecule fluorometry comprising; the first step wherein, in a test solution containing a nucleic acid extract prepared from a biological sample to which the siRNA has been administered, dissociating the double strands of the siRNA and ...
Fig.4.Starvio siRNA transfection reagent offers consistent high level of gene knockdown despite differences in cell density.A549 cells at different cell density were transfected with Lamin siRNA (final concentration: 10nM) using Starvio. 适用细胞 293T(人肾上皮细胞),A549(人肺 癌细胞),HEK 293...
Minimize sequence-specific off-targeting by lowering the relative concentration of each siRNA Better mimic the natural RNAi pathway Reduce false negatives caused by inaccessible binding sitesWhich siRNA is right for you? Horizon offers multiple predesigned product lines across human, mouse and rat genomes...
the cells were transfected 24 hours after plating, using 2 µl siport lipid (ambion) according to the manufacturer's protocol, at a final sirna concentration of 75 nm. immunofluorescence analysis was performed 96 hr post transfection using mouse anti-human ß-actin prim 9、ary antibody and...
转染后无需去除转染复合物或者更换培养基。 测试推荐的两种浓度的LipofectamineTM 3000试剂,以确定最佳用量,开始新的转染。 实验步骤: 1. 接种细胞,使其在转染时达到70-90%汇合度。 2. 制备质粒DNA-脂质体复合物(推荐两种剂量的脂质体) 3. 加入DNA-脂质体复合物至细胞中。
1ulsiRNA 3ulsiRNA B.siRNAmix 110nM2Neo10nM3Sb4Sb5N26N2 75ulOPT+siRNA 1- 3Sb 5N2 2Neo 4Sb30nM 6N230nM Step2preparesiRNAvehiclecomplexbymix75ulAtoB(75ul)=150ul,thenincubate10minRT 75ulComplex150ul75ul 12Neo3Sb4Sb5N26N2 ••• Countcell,andpreparecelltomakefinalconcentration0.1Million...