Three human-specific mtDNA targets of increasing length (86, 190 and 452 base pairs) were amplified by singleplex quantitative real-time PCR (qPCR), capable of providing an index of mtDNA degradation from fragment length information. Quantification was achieved by preparation of a standard curve ...
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Moreover, we show that the qPCR method for telomere length estimation can be highly precise and accurate, and thus suitable for telomere measurement in non-model species, if effort is devoted to optimization at all experimental and analytical steps. We conclude by highlighting a set of quality ...
For this study, a two-step real-time PCR (qPCR) was performed by using the fluorophore FAM based on the TaqMan® Protein Assays Probe Development Protocol (LifeTechnologies, Applied Biosystems, Austria). Proseek reagents have been prepared according to the Proseek user manual (Olink, Bioscience...
【PCR/RT-PCR/qPCR】PCR试剂|PCR对照|特异性PCR试剂盒|PCR克隆试剂盒|RNA 载体及构建|PCR克隆载体|M13克隆载体|克隆载体|文库及构建|cDNA文库 基因组文库 噬菌体展示文库【蛋白检测】Western印迹|报告基因检测|蛋白检测试剂盒 【克隆与表达】克隆基因|克隆试剂盒|克隆筛选【核酸/蛋白合成】Oligo纯化|核酸合成柱|核酸...
685. Comparison of Singleplex qPCR and the Luminex MAGPIX Platform for the Detection of Viral PathogensErin Yepsen, BSZaid Haddadin, MDDanielle A Rankin, MPH, CICRendie McHenry, MSLaura S Stewart, PhDNatasha B Halasa, MD, MPHOpen Forum Infectious Diseases...
【PCR/RT-PCR/qPCR】PCR试剂|PCR对照|特异性PCR试剂盒|PCR克隆试剂盒|RNA 载体及构建|PCR克隆载体|M13克隆载体|克隆载体|文库及构建|cDNA文库 基因组文库 噬菌体展示文库【蛋白检测】Western印迹|报告基因检测|蛋白检测试剂盒 【克隆与表达】克隆基因|克隆试剂盒|克隆筛选【核酸/蛋白合成】Oligo纯化|核酸合成柱|核酸...