Real-time PCRTarget Enriched Multiplex PCRMolecular diagnostic devices are increasingly finding utility in clinical laboratories. Demonstration of the effectiveness of these devices is dependent upon comparing results from clinical samples tested with the new device to an alternative testing method. The ...
Sample processing (PCR settings, cycle conditions and detection), results interpretation (controls and Ct cut off for positive samples), and LoD were all maintained the same from the singleplex assay to the multiplex sample strategy. Pooled assay assessment To create pooled samples with known stat...
A few sets of primers and probes targeting several genomic regions can be mixed together in one PCR reaction to produce multiplex PCR. This multiplexing, however, comes with multiple challenges such as varying efficiency of individual assays, different primer annealing temperatures, possible ...
In assay II, we tested the multiplex approach presented by Cawthon [24]. Here, telomere primers have been designed to amplify a fixed-length PCR product with well-defined melting profile useful for subsequent evaluation, and the reference gene primers contain a CG-clamp which increases their me...
real-time RT-PCRvirusesSingleplex and multiplex real-time (TaqMan) RT-PCR assays were developed to detect seven fig-infecting viruses, i.e., fig leaf mottle-associated virus 1 (FLMaV-1), fig leaf mottle-associated virus 2 (FLMaV-2), fig mild mottle-associated virus (FMMaV), fig ...
It was observed that through both PCR methods the fingerprinting profile of a subset of samples was identical. The genetic analyses revealed that non-Arabica coffee displayed greater genetic variation than Arabica coffee did. In addition, microsatellite analyses allowed the...
RT-PCRReal-time RT-PCRTaqMan®Singleplex and multiplex real-time (TaqMan) RT-PCR assays were developed to detect seven fig-infecting viruses, i.e., fig leaf mottle-associated virus 1 (FLMaV-1), fig leaf mottle-associated virus 2 (FLMaV-2), fig mild mottle-associated virus (FMMaV),...
Sakthivel SK, Whitaker B, Lu X, et al. Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT- PCR assay with in-house singleplex assays for comprehen- sive detection of human respiratory viruses. J Virol Methods. 2012;185:259‐266....
Liquid biopsies offer the potential to monitor cancer response and resistance to therapeutics in near real-time. However, the plasma cell free DNA (cfDNA) level can be low and the fraction of circulating tumour DNA (ctDNA) bearing a mutation – lower sti
Specifically, when singleplex PCR results exceeded 250 copies/mL for DNA viruses and 500 copies/mL for the RNA virus, the concordance rate with the QIA/ME was 96.8%. In contrast, when PCR values were below these thresholds, the concordance rate dropped to 43.8%. A strong overall correlation...