By the nature of PCR, its two primers (and optionally a probe) target a very specific sequence. A few sets of primers and probes targeting several genomic regions can be mixed together in one PCR reaction to produce multiplex PCR. This multiplexing, however, comes with multiple challenges ...
Liquid biopsies offer the potential to monitor cancer response and resistance to therapeutics in near real-time. However, the plasma cell free DNA (cfDNA) level can be low and the fraction of circulating tumour DNA (ctDNA) bearing a mutation – lower sti
In assay II, we tested the multiplex approach presented by Cawthon [24]. Here, telomere primers have been designed to amplify a fixed-length PCR product with well-defined melting profile useful for subsequent evaluation, and the reference gene primers contain a CG-clamp which increases their me...
In contrast, when PCR values were below these thresholds, the concordance rate dropped to 43.8%. A strong overall correlation was observed between the viral load measured by singleplex PCR and Ct values from the QIA/ME (蟻 = 0.83, p < 0.001). Only for enterovirus a weak correlation was ...
(DNA-template gradients vs. # PCR cycles), (C) to co-amplify conserved regions of the prokaryotic 16S rRNA using “universal primers” to switch from OD/AU to “normalized units” (semiquantification), (D) to migrate this “single plex” endpoint 2S-PCR protocol to a multiplex assay and...