Statistics of single-molecule measurements: applications in flow-cytometry siz- ing of DNA fragments. Cytometry Part 1 60, 41-52.Ferris MM, Habbersett RC, Wolinsky M, Jett JH, Yoshida TM, Keller RA. Statistics of single-molecule measurements: Applications in flow- cytometry sizing of DNA ...
Single-molecule long-read sequencing technology (third-generation sequencing; PacBio Iso-Seq, Supplementary Fig. 1b) provides a means to identify full-length transcripts34. The PacBio system can produce long reads (up to 30 kb vs 250 bp for short-read sequencing). Although long-read sequ...
Controlled release of single-molecule cytosolic proteins We first asked whether a soluble molecule could be confined to a specific location in the cell and released following photocleavage of PhoCl. To do so we made use of FGF2, a cytosolic protein secreted via an unconventional pathway through ...
interpretation of accessible regions with respect to enhancer–promoter proximity, functional transcription factor binding and regulatory function. We envision that technological improvements including single-molecule, multi-omics and spatial methods will bring further insight into the secrets of genome ...
We report a study on protein−protein noncovalent interactions in an intracellular signaling protein complex, using single-molecule spectroscopy and molecular dynamics (MD) simulations. A Wiskott−Aldrich Syndrome Protein (WASP) fragment that binds only the activated intracellular signaling protein Cdc42...
BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Frank,Diehl,Meng,... - 《Nature Methods》 被引量: 455发表: 2006年 Single-molecule PCR on microparticles in water-in-oil emulsions BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Nature Methods....
Theoretical investigation of aspects of single‐molecule fluorescence detection in microcapillaries In the present report, the results of a theoretical investigation of two aspects of single-molecule detection by laser-induced fluorescence in microcapilla... J Enderlein - 《Cytometry》 被引量: 18发表:...
2.4. Single-molecule RNA in situ hybridization (RNAscope) We used in situ hybridization via RNAscope probes in E16.5 mouse mandibular molar tooth germs and the Plac8 positive regions, which were carefully dissected from the tooth mesenchyme using two needles under the microscope. We performed tiss...
Single-molecule imaging goes high throughputCombining single-molecule localization microscopy with flow cytometry and three-dimensional localization enables the high throughput and precise localization of fluorescent molecules in live flowing cells.doi:10.1038/s41565-020-0676-7Jrg Enderlein...
This appears to involve stochastic processes when amplifying a single DNA molecule as a starting template, as well as a bias against sequences with high G + C content31. Recent work has shown that co-assembly of eukaryote SAGs from several cells of the same population significantly ...