Cytometry performed by suspending cells in a liquid and passing them through a light beam, often after applying fluorescent stains. The measured characteristics of the scattered light correlate with cell properties such as volume, morphology, and pigmentation. ...
flow cytometry a cytometric technique in which cells suspended in a fluid flow one at a time through a focus of exciting light, which is scattered in patterns characteristic to the cells and their components; cells are frequently labeled with fluorescent markers so that light is first absorbed an...
Flow cytometry was performed on a BD Accuri C6 with manufacturer standard fluorescence detector set-up (FL1, 533/30 nm; FL2, 585/40 nm; FL3, > 670 nm; FL4, 675/25 nm) and data acquisition with BD Accuri C6 software including recording processed sample volume. Quality assurance was...
During the period of study, flow cytometry of DNA content was performed on bone marrows of 115 adult patients with acute leukemia. Eighty patients—acute myeloblastic leukemia (AML), 56; acute undifferentiated leukemia (AUL), 5; acute lymphoblastic leukemia (ALL), 15; and lymphoma leukemia (LL...
Estimation of Cell Viability by Flow Cytometry 来自 NCBI 喜欢 0 阅读量: 28 作者: JL Weaver 摘要: The estimation of the viability of a cell population by flow cytometry is based on a simple yet powerful principle: Dead cells leak. Cells that die via the necrosis pathway, in contrast ...
(C) Deformability cytometry. (D) Electronically triggered deformability cytometry with on-demand imaging in viscoelastic fluids. (E) Pinched-flow hydrodynamic stretching. (F) Virtual fluidic channel deformability cytometry.(Part A: Otto, O.; Rosendahl, P.; Mietke, A.; Golfier, S.; Herold, C...
Add 10% FBS to termination and washing intima with PBS to separate the endothelial cells. PBS was centrifuged and endothelial cells were collected. The proportion of endothelial cells was tested by flow cytometry.RNA isolation and RT-qPCR Total RNA was extracted from different treated p-HUVECs ...
16. The method according to claim 1, wherein said fluorescence measuring step (f) at least in part is performed by a flow cytometry. 17. The method according to claim 1, wherein the non-mutated genetic sequence of the nucleic acid includes a partially non-mutated sequence. ...
irradiation of unshaped light beam, the cells in the flow cytometer will not cause signal attenuation even when they deviate from the central axis of the channel or the main axis of the light beam as they flow in the channel, which is thus more suitable for high-throughput flow cytometry. ...
Next, a typical flow cytometry system (“flow cytometer”) will be described. A typical flow cytometer includes a laser light irradiation optical system, a flow chamber, a detection optical system, and a control unit. The laser light irradiation optical system radiates laser light onto micropartic...