Jiang’s not alone in embracing multi-dimensional profiling approaches in single cell experiments. Jonas Schulte-Schrepping from the University of Bonn, Germany, and colleagues recently described how high-parameter flow cytometry, along with scRNA-seq and cytometry by time of flight, could be used ...
Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria David A. Barr, Charles Omollo, Mandy Mason, Anastasia Koch, Robert J. Wilkinson, David G. Lalloo, Graeme Meintjes, Valerie Mizrahi, Digby F. Warner & Gerry Davies Scientific Reports volume 11,...
The process for preparing a single-cell suspension is largely determined by the origin of the cells. Lauren Jachimowicz, application development scientist, flow cytometry at Agilent Technologies, notes that cultured cell lines can readily be transformed into a single-cell suspension ...
Single-cell-analysis-flow-cytometry:为了揭示流式细胞仪数据内的细胞异质性,可以进行聚类和伪时间分析。 在这里,我们演示了如何导入(聚类)数据,如何应用聚类和降维以及Tr**re 上传149.71 MB 文件格式 zip HTML 单细胞分析流式细胞仪 为了揭示流式细胞仪数据内的细胞异质性,可以进行聚类分析和伪时间分析。 可视化...
Further downstream applications of the sorted cells include cell culture, RNA-sequencing, and proteomic or metabolite analysis. Cell Cycle Analysis Cell cycle analysis was one of the earliest applications of flow cytometry. It uses fluorescently labelled DNA probes that bind stoichiometrically to DNA (...
Flow cytometry is used to monitor changes in cell-surface and intracellular proteins, but some proteins such as cytokines may be transient and difficult to measure. Thus, a flow cytometry method that can simultaneously measure cytokine mRNA and protein levels in single cells is a very powerful ...
AS Waggoner - 《Flow Cytometry & Sorting》 被引量: 91发表: 1990年 [Principles of flow cytometry] The measurement of DNA content by flow cytometry is widely used for the evaluation of proliferative activity in single cell suspensions. The simultaneous r... D Grunwald - 《Bulletin Du Cancer》...
Single cell phospho-specific flow cytometry (SCPFC) enables the investigation of signaling network interactions and the categorization of disease outcome. While this method has been successfully used to study hematologic disorders, its application on solid tumors has not been examined. This study aimed...
Flow cytometry, the methodology that provides a way to measure physical and chemical attributes of individual cells rapidly and with high accuracy, is wide... Gray,W J. 被引量: 0发表: 1979年 Analysis of Cell Cycle by Flow Cytometry Described are four widely used procedures to analyze the ce...
Calculating compensation requires controls including unstained, fluorescence minus one (FMOs), and single-color samples. Learn more about basics of panel design and flow cytometry controls. Beads are recommended when: Multiple fluorophore emis...