The invention provides engineered heteromultimeric protein complexes constructed using one, two, or three tethers and methods for making, using, and purifying such complexes, such as antibodies with different binding properties.B·弗拉尼克D·L·伊顿E·H·克里斯腾森J·吴...
Single-chain antibodies against human insulin-like growth factor I receptor: expression, purification, and effect on tumor growth. Insulin-like growth factors (IGF) I and II are potent mitogens for a variety of cancer cells. The proliferative and anti-apoptotic actions of IGF are media... SL...
Klausner, A., Single Chain Antibodies Become a Reality, Bio/Technology 4 :1041, 1043 (Dec. 1986).Klausner, A., " Single-Chain Antibodies Become a Reality, " Bio/Technology 4:1041, 1043 (Dec. 1986).Klausner, A., Single Chain Antibodies Become a Reality, Bio/Technology 4 :1041,1043 (...
no licensed vaccine or effective therapy is presently available for human use. Single chain antibodies (VHH) produced by camelids exhibit several advantages
Ras and p53 intracellular targeting with recombinant single-chain Fv (scFv) fragments: a novel approach for cancer therapy? Intracellular expression of recombinant antibodies allows one to interfere with the functions of oncogenic molecules expressed in various cell compartments and has therefore a vast ...
In the last two decades, with the advent of recombinant DNA technology, various forms of recombinant antibodies have been generated [2], [3]. These include chimeric and humanized antibodies as well as antibody fragments such as Fab, F(ab)′2 and single chain variable fragment antibodies (scFv...
所有这些形式都可以由对两种不同抗原具有特异性的可变片段组成,在这种情况下它们是双特异性抗体(bispecific antibodies,后文有详细内容)的类型。 其中研究的最深的是双特异性串联di-scFv,称为双特异性T细胞接合者( bi-specific T-cell engagers ,BiTE抗体构建体)(翻译自https://en.wikipedia.org/wiki/Single-chain...
9.1Single chain antibodies (ScFv) Recombinant antibody(rAb) fragments are becoming popular therapeutic alternatives to full lengthmonoclonal Abssince they are smaller, possess different properties that are advantageous in certain medical applications, can be produced more economically and are...
Antibodies targeting the ErbB-2 pathway are a preferred therapeutic option for patients with advanced breast cancer, but a worldwide deficit in the manufacturing capacities of mammalian cell bioreactors is foreseen. Methods Herein, we describe a multi-platform approach for the production of recombinant...
Sera of camelids contain both conventional heterotetrameric antibodies and unique functional heavy (H)-chain antibodies (HCAbs). The H chain of these homodimeric antibodies consists of one antigen-binding domain, the VHH, and two constant domains. HCAbs fail to incorporate light (L) chains owin...