Single-cell western blotting(单细胞免疫印迹) 下载积分: 3000 内容提示: Single-cell western blottingAlex J. Hughes 1,2,6,7 , Dawn P. Spelke 1,2,3,7 , Zhuchen Xu 1,2 , Chi-Chih Kang 1,2 , David V. Schaffer 1,2,3,4,5 , and Amy E. Herr 1,2,31 Department of Bioengineering,...
A microscope slide supporting a 30-m-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into microwells, lysis in situ, gel electrophoresis, photoinitiated blotting to immobilize proteins and antibody probing. We applied this scWestern method to monitor single-cell ...
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct 10(3) concurrent single-cell western blots (scWesterns) in 4 h. A microscope slide supporting a 30-m-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into ...
DropBlot: single-cell western blotting of chemically fixed cancer cells Yang Liu Amy E. Herr Nature Communications(2024) Integrating organoids and organ-on-a-chip devices Yimu Zhao Shira Landau Milica Radisic Nature Reviews Bioengineering(2024) ...
However, the enriched cell suspension usually still contains a large number of background cells (erythrocytes and leukocytes), making it difficult for downstream analysis. Although fluorescent staining with immunohistochemistry (IHC), in situ hybridization (ISH), and single-cell western blotting may be...
6B-D). Next, we found the treatment of U73122 in THP-1 and Molm-13 cells resulted in an increase in the percentage of cells in the G1 phase and a decrease in S phase, indicating cell cycle G1/S arrest (Fig. 5F; supplemental Fig. S5C). Corresponding to that, western blotting ...
size (bottom). f. The expression ofTPSAB1in human CRC tissue and paired NC tissue by Western blotting. g. Immunofluorescence staining of human CRC tissue and paired NC tissue.TPSAB1(pink),CMA1(green),CPA3(green), DAPI (blue), Bar, 200 μm. CRC, colorectal cancer; DEGs, differential...
黑色素瘤细胞系A2058是一个2G纯合体,具有高水平的MMP-1组成型表达.Tower et al[172]利用Northern blotting,Western blotting,荧光素酶活性分析和PCR为基础的定点突变方法研究发现,如果加入一个针对ERK途径的特异性抑制剂PD098059,则MMP-1的表达受阻,由此可见ERK1/2途径主要以MMP-1的2G多态型为靶点,促进MMP-1基因...
Western blotting of human testis Twenty milligrams of human testis and human muscle tissues were lysed, respectively, with 1 ml lysis buffer composed of 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 0.5 mM DTT, and 0.4 U/μl of Proteinase inhibitor ...
Despite achieving great advancements in exploring the mechanism of tumor-immune interplay, traditional techniques, such as western blotting (WB), coimmunoprecipitation (Co-IP), and real-time quantitative polymerase chain reaction (RT-qPCR), cannot provide a thorough landscape of the TIME. There is ...