二抗:Anti-rabbit IgG (H+L) (DyLight™680 Conjugate)近红外荧光抗体(Cell Signaling Technology,5366),使用Cell Signaling Technology配套荧光抗体稀释液TrueBlack®Fluorescent Western Blot Blocking Buffer Kit(Cell Signaling Technol...
Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. At Cell Signaling Technology (CST) we understand that western blotting experiments are time consuming and that their success has a critical impact on your research progress. For that reason, we...
本次研讨会将通过介绍CST Western Blot标准操作步骤,向您阐释WB操作中的关键点及注意事项,特别是影响最终结果呈现的样本制备和抗体孵育与检测。通过真实失败案例,如无信号、高背景、条带大小不正确、条带弯曲拖尾等,分析WB常见的问题及原因,并提供相应的解决策略。About Us Our Company Our Approach/Process Product...
Single-Cell Western usesconventional Western blot antibodiesand is capable of resolving phosphorylated isoforms that are critical for understanding the activity of cell signaling networks. Taken together with Simple Western, these advanced Western blotting solutions offer protein characterization at both a pop...
1Cell Signaling Technology, Inc.Abstract The western blotting technique measures protein expression in a cell or tissue extract using antibody-based detection of a target protein. The antibodies used in a western blot can be specific to a total protein or to unique post-translational modifications ...
无载体和定制配方Cell Signaling Technology (CST)提供了全面的无载体配方抗体(PBS中无BSA,无甘油和无叠氮化合物),这些抗体适用于诸如:用于CyTOF质量细胞术的类ELISA测定如AlphaLISA,HTRF和MSD测定的金属标记,成像质量细胞测定TM (IMC)和用于流动和免疫荧光的缀合的MIBIO寡核苷酸标记荧光染料在体内和功能测定中,这些无载...
Key to the regulation of the G protein signaling cascade is the involvement of cyclic AMP (cAMP). One of its key functions is to activate Guanine Exchange Factors (GEF), which in turn play a role in the activation of G proteins. Small GTPases serve to regulate a variety of cell signali...
sepsis. Our results, therefore, establish a direct link between TLR4 signaling and ACOD1 expression through the STING1-MYD88 complex during septic shock. ACOD1(也称为 IRG1)已成为免疫代谢的调节剂,通过产生代谢物衣康酸来发挥作用。在这里,我们报告了 STING1(也称为 STING 和 TMEM173)在响应 toll...
答:请参考CST网站上该产品的Western blot图,使用图述的细胞系和药物处理,能够得到阳性对照蛋白。也可参见:www.cellsignal.com/support/controls.html查阅。 7.问:假如说明书上某个种属未被列出与CST抗体有交叉反应,是否意味着这个种属未被检测过呢? 答:如果可能的话,CST科学家检测所有CST抗体在人、小鼠和大鼠的交...
Cell Signaling Technology (CST) 是美国著名的生物公司和细胞信号转导研究的领袖,提供特色的信号转导检测用相关产品,用于细胞功能和疾病发病机理的探讨。Cell Signaling Technology正在招聘Technical Application Specialist /技术应用支持,欢迎应聘。 Cell Signaling Technology (CST) 是美国著名的生物公司和细胞信号转导研究...