Single-cell western blotting(单细胞免疫印迹) 下载积分: 3000 内容提示: Single-cell western blottingAlex J. Hughes 1,2,6,7 , Dawn P. Spelke 1,2,3,7 , Zhuchen Xu 1,2 , Chi-Chih Kang 1,2 , David V. Schaffer 1,2,3,4,5 , and Amy E. Herr 1,2,31 Department of Bioengineering,...
This protocol describes how to perform western blotting on individual cells to measure cell-to-cell variation in protein expression levels and protein state. Like conventional western blotting, single-cell western blotting (scWB) is particularly useful for protein targets that lack selective antibodies ...
To address these protein analysis gaps, we have introduced a single-cell western blot (scWestern). The protein assay is capable of highly specific analysis by coupling antibody-based detection with a polyacrylamide gel electrophoresis (PAGE) protein separation. Cells are settled via gravity into ...
For detection of this cell-to-cell variation, a highly advanced technique termed “single-cell western blotting” is carried out. Single-cell western blot has enabled us to detect protein expression at cellular level at a fairly advanced high resolution using a western blot designed to assess ...
To measure cell-to-cell variation in protein-mediated functions, we developed an approach to conduct 10(3) concurrent single-cell western blots (scWesterns) in 4 h. A microscope slide supporting a 30-m-thick photoactive polyacrylamide gel enables western blotting: settling of single cells into ...
Archived patient-derived tissue specimens play a central role in understanding disease and developing therapies. To address specificity and sensitivity shortcomings of existing single-cell resolution proteoform analysis tools, we introduce a hybrid micro
https://singlecell.broadinstitute.org/single_cell进入主页: 可以看到数据库为我们提供了两种检索模式,一种是根据研究项目检索,一种是根据基因进行检索。这两检索模式只是检索方式路径不同,我们主要介绍一下按研究项目检索: 首先根据所需筛选研究项目,比如我们想看人的肺纤维化组织的单细胞数据,设置筛选条件(物种智人...
In vivo single-cell CRISPR screening To monitor tissue-wide clonal expansion using an in vivo single-cell CRISPR strategy, we adjusted the CRISPR droplet sequencing (CROP-seq) system5,6by substituting the puromycin cassette with mCherry to enable selection of infected cells by fluorescence-activated...
suggesting their myeloid origin. We validate this finding using single-cell western blot and single-cell RNA-sequencing on genetically labeled myofibroblasts. Using bone marrow transplantation and Cre recombinase-based lineage tracing experiments, we rule out cell fusion events and confirm that hematopoieti...
Highly multiplexed, single-cell technologies may be critical for identifying correlates of disease or immunological interventions as well as for elucidating the underlying mechanisms of immunity. Here we review limitations of bulk measurements and explore advances in single-cell technologies that overcome ...