Preparation of a single cell suspension from solid tissue is vital for a successful flow cytometry experiment. We report a detailed and reproducible method to produce a quality cell suspension from the zebrafish retina. Zebrafish retinas, especially their M眉ller glia cells, are of particular ...
By using this method, the difficulty of the procedure was reduced, fewer cells were lost during the preparation treatments, and the maximal activity of single cells was maintained. Following preparation of the cell suspension, the HSCs were further isolated using a Nycodenz density gradient. Cell ...
Single-cell analysisSingle-cell suspensionSolid tissueTissue digestionDue to its technological advantages, single-cell sequencing has become an increasingly utilized tool to unravel the heterogeneity and complexity of individuals. The preparation of a high-quality single-cell suspension from solid tissues ...
4. Place 20 to 50 μL of the cell suspension (at least enough to cover the well) in each well of the adhesion slides and let cells adhere at room temperature (RT) for 20 min. 5. Continue with the staining procedure. Centrifugation method 1. Assemble the CytoSpin centrifuge's sample ...
Goldman, W.F., Stefano, A.D., Allen, M.D.et al.Preparation of suspensions of single cells from monolayer cell cultures without enzymatic dissociation.Tca Manual5, 1135–1136 (1979). https://doi.org/10.1007/BF00920006 Download citation ...
Cell source dictates sample-preparation methods The process for preparing a single-cell suspension is largely determined by the origin of the cells. Lauren Jachimowicz, application development scientist, flow cytometry at Agilent Technologies, notes that cultured cell lines can readily be...
Single-cell suspension preparation Bladder tumors and adjacent normal mucosa were processed immediately after being obtained from bladder cancer patients. Every sample was cut into small pieces (<1 mm in diameter) and then was incubated with 2 ml of trypsin (Gibco, Cat: R001100), 1 ml of ...
Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible for cell reprogramming during somatic embryogenesis are still largely unclear. Recent advances in single-cell technol
which needed to be above 85% and single-cell suspensions were adjusted in concentration to 700–1200 cells/μl. After completing the tissue digestion and single-cell suspension preparation, as indicated by the 10×Genomics pre-sequencing needed, we followed by cell sorting, reverse transcription, ...
while surgical biopsy samples were from young and middle-aged individuals undergoing surgery for intractable epilepsy. The single-cell suspension preparation of sorted cells was loaded onto one lane of the Chromium system (10x Genomics) and the resulting library was sequenced on the HiSeq4000 platform...