If you want to perform imaging of adherent cells, such as to undertakecancer cell microscopy, you need to fix them to your microscope slides. Luckily for you, this is fairly easy to do, thanks to the fact that adherent cells are, well, adherent, and like to grow on plastic or glass—...
In this application note we describe how to stain cells for dynamic cell culture in a perfusion chamber with different microfluidic setups.
If an automated system is not available in your facility, manual cell counting can be performed using a reusable or disposable hemocytometer, which has two chambers etched with grids. The cell suspension is drawn into the chambers by capillary action between the grid and the coverglass, forming ...
Importantly, the sexually dimorphic brain, similar to most sex differences, does not fall into a hard binary readout—but rather is on a continuum or spectrum with each cell and each brain region comprised of varying degrees of 'male' and 'female' (Hines, 2005; Joel and McCarthy, 2016)....
An alternative approach involves transforming cells derived from suspension cultures of citrus embryogenic calli (Omar et al.2016,2018; Dutt et al.2020). The use of cationic lipid-based carriers, such as Lipofectamine, provides a viable method for delivering donor DNA and achieving successful genome...
(IF) at the desired time. Immunofluorescence (IF) is also possible with suspension cells after application of the cells to an object slide, e.g. by cytospin. In both cases, the focus is on the analysis of intracellular processes or structures, and this is designated immunocytochemistry (ICC)...
(1880) and Klercker used the very first mechanical method to protoplast isolation (1892). However, the greatest development of protoplast isolation occurred in 1960 when the first enzymatic method for the cell wall removal has been introduced by Cocking. Up to this time, protoplasts have been ...
The stem cell lines he claimed to have derived did not exist, and the research data was heavily manipulated or completely fabricated. The scientific community and the public were shocked by the extent of the deception. As a result, the scientific community lost trust in Dr. Hwang's work, ...
Add a multi-buy offer.Increaseaverage order valueusing multi-buy offers, such as one product for $5 or three for $12. This can increase the profitability of your online business since there’s no need to pay shipping or import fees several times. ...
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