Whole-slide, spatial phenotyping was performed using the PhenoCycler -Fusion system, followed by deep bioinformatic analyses to determine the cellular phenotypes, spatial neighborhoods, and metabolic determinants of SLE. By combining immune cell lineage information with expression of key proteins involved ...
此外,在血液中发现了一个罕见的CCR9+T细胞亚群,它具有组织归巢特性和几种免疫检查点分子的表达,提示其具有调节功能。 二、样本制备处理细节 不刺激组: 冻存PBMC:复苏冻存的PBMC 磁珠分选CD4+T CELLS 同时孵育流式抗体和Abseq抗体,之后进行流式分选 样本混样:采用BD Rhapsody的混样试剂盒(633781 BD single cell ...
Non-coding genetic variants may cause disease by modulating gene expression. However, identifying these expression quantitative trait loci (eQTLs) is complicated by differences in gene regulation across fluid functional cell states within cell types. The
The immune cell composition and gene expression profiles in pregnant SLE patients, as well as the molecular mechanisms of active SLE patients during pregnancy, remain unclear. In our study, we enrolled 12 patients: three active SLE individuals (SLE-AT group, SLEDAI > 12, non-pregnant women), ...
single-cell RNA sequencing UMI: unique molecular identifiers IFCs: Integrated Fluidic Circuits NGS: next-generation sequencing ddPCR: droplet digital PCR IFN-α: interferon alpha IPC: IFN-α producing cells LN: lupus nephritis SLE: systemic lupus erythematosus RCC: renal cell carcinoma ...
Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with different degrees of disease activity and 11 matched controls. Increased expression of interferon-stimulated genes (ISGs) distinguished cells from children with SLE from healthy ...
Alternative splicing contributes to complex traits, but whether this differs in trait-relevant cell types across diverse genetic ancestries is unclear. Here we describe cell-type-specific, sex-biased and ancestry-biased alternative splicing in ~1 M pe
The scRNA-seq results of peripheral blood cells from five different autoimmune diseases (IgAN, KD, MS, SS, and SLE) were integrated. All samples contained 18 different immune cell subsets, although the cell cluster populations were different among the five diseases. ...
Single cell RNA sequencing to dissect the molecular heterogeneity in lupus nephritis. JCI Insight 2, e93009 (2017). The first scRNA-seq study in SLE, showing upregulation of ISGs in skin keratinocytes and renal tubules of patients. Article PubMed PubMed Central Google Scholar Mizoguchi, F. ...
aberrant B-cell response. As another example, enrichment analysis identifies B cells as the most relevant cell type for SLE. SLE is characterized by B cell dysfunction that results in the production of pathogenic autoantibodies. SLE B cells also possess altered antigen presentation and cytokine secre...