Whole-slide, spatial phenotyping was performed using the PhenoCycler -Fusion system, followed by deep bioinformatic analyses to determine the cellular phenotypes, spatial neighborhoods, and metabolic determinants of SLE. By combining immune cell lineage information with expression of key proteins involved ...
Single cell RNA sequencing to dissect the molecular heterogeneity in lupus nephritis. JCI Insight 2, e93009 (2017). The first scRNA-seq study in SLE, showing upregulation of ISGs in skin keratinocytes and renal tubules of patients. Article PubMed PubMed Central Google Scholar Mizoguchi, F. ...
The kidney is an important organ for maintaining normal metabolism and stabilising the internal environment, in which, the heterogeneity of cell types has hindered the progress in understanding the mechanisms underlying kidney disease. In recent years the application of single-cell RNA sequencing (scRNA...
Non-coding genetic variants may cause disease by modulating gene expression. However, identifying these expression quantitative trait loci (eQTLs) is complicated by differences in gene regulation across fluid functional cell states within cell types. The
To delineate the cellular and molecular basis for DN initiation, we performed single-cell and bulk RNA sequencing of renal cells from type 2 diabetes mice (BTBRob/ob) at the early stage of DN. Results Analysis of differentially expressed genes revealed glucose-independent responses in glomerular ...
Alternative splicing contributes to complex traits, but whether this differs in trait-relevant cell types across diverse genetic ancestries is unclear. Here we describe cell-type-specific, sex-biased and ancestry-biased alternative splicing in ~1 M pe
The immune cell composition and gene expression profiles in pregnant SLE patients, as well as the molecular mechanisms of active SLE patients during pregnancy, remain unclear. In our study, we enrolled 12 patients: three active SLE individuals (SLE-AT group, SLEDAI > 12, non-pregnant women), ...
Sequencing costs currently prohibit the application of single-cell mRNA-seq to many biological and clinical analyses. Targeted single-cell mRNA-sequencing reduces sequencing costs by profiling reduced gene sets that capture biological information with a
Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with different degrees of disease activity and 11 matched...
典型的Th1(TBX21,编码TBET)和Th17(RORC,编码RORγt)谱系定义转录因子的表达仅限于记忆Teff(mTeff)群体中的特定簇,表明这些簇高度富集Th1和Th17 Teffs。并观察到转录因子的表达梯度,揭示了该SLE患者血液中高度激活的Th1 T细胞亚群具有假定的细胞毒性。Th17特征基因,包括RORC的表达梯度表明Th17分化的轨迹。 5. 单细...