SinglecellRNA-seqdataanalysiswithR简介和实验设计质量控制数据预处理归一化和批处理效果校正数据集成ccamnn数据集对齐降维pcatsne和umap聚类差异基因表达分析细胞类型识别轨迹伪时间空间转录组学 SinglecellRNA-seqdataanalysiswithR CSC – IT科学中心是芬兰国家和高等教育机构拥有的芬兰信息技术专业中心。为高等教育机构,...
练习地址:https://github.com/NBISweden/excelerate-scRNAseq/blob/master/session-clustering/Clustering.md 之前讲了质量控制、降维,现在这一步就是鉴定你的data里的细胞群。上图就是一个简单的流程图。拿到scRNA-seq 的matrix,这个matrix里含有细胞和features(这里就是基因)。然后你把细胞聚类(clustering),然后你再试...
In single cell sequencing, usually ,only cells between cells normalization usually done with 3' prime tag data. You don't need to do the gene length normalization. But if you have full length data, like from SMART-seq2, then also the gene length must be take into account. So the aim ...
单细胞RNA测序Single Cell RNA Sequencing vs. Bulk RNA Sequencing 3876 1 7:35:42 App 生肉|Single cell RNA-seq data analysis with R (2019) 单细胞RNA测序数据分析教程 2万 31 31:32 App 30分钟拿下RNA-seq原理、操作方法、分析方法和应用场景 443 -- 3:15:30 App 单细胞RNA-seq ChiPster 6599...
从这个系列开始，我们将跟着一篇《Cell》文章学习单细胞转录组数据的下游分析，以及下游分析数据的个性化...
图片来源:Zhang M J, Hou K, Dey K K, et al. Polygenic enrichment distinguishes disease associations of individual cells in single-cell RNA-seq data[R]. Nature Publishing Group, 2022. 2、通过scDRS分析可以得到什么 第一、鉴定表型相关的细胞类型,比如下图: ...
Introduction R/Bioconductor Expression QC and Normalization Data Wrangling scRNAseq Identifying Cell Populations Feature Selection and Cluster Analysis Batch Effects Correcting Batch Effects Functional Analysis Pseudotime Cell Trajectories Functional Pseudotime Analysis Single Cell Multiomic Technologies CITE-seq and...
theory based on the popular splicing analysis method rna velocity will also be demonstrated. finally, we will discuss our recent efforts to interpolate non-stationary time-series scrna-seq data using wasserstein-fisher-rao-metric unbala...
[论文笔记] :MetaCell: analysis of single-cell RNA-seq data using K-nn graph partitions 论文是一种非参数方法将细胞分为不相交的细胞团,同时避免数据平滑 整个算法流程为: 1. 原始矩阵进行特征选择 2. 构建平衡KNN图:如果我们用传统的相似性网络作为基因的co-expr网络的时候,每个节点根据阈值的选择,不同...
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has advanced our understanding of the pathogenesis of disease and provided valuable insights into new diagnostic and therapeutic strategies. With the expansion of capacity f