Learn how Milo detects targets in single-cell sequencing runs and provides single-cell protein expression information to validate single-cell RNA-seq data.
Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive analysis of the immune system. In this Review, we briefly describe the available scRNA-seq technologies together with their corresponding strengths and weaknesses. We discuss in depth how scRNA-seq can be used to de...
Two new techniques now available to explore such inter-cellular transcriptomic diversity are single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics. This chapter reviews the computational analysis involved in scRNA-seq studies. We list and describe the computational algorithms and software ...
Single cell analysis & RNA sequencing kits. Bring the power of single-cell biology to your lab with instrument free scRNA-seq solutions.
5. To identify these celltype-specific eQTLs using an unbiased approach, we used single-cell RNA sequencing to generate expression profiles of ~25,000 peripheral blood mononuclear cells from 45 donors. We identified previously reported cis-eQTLs, but also identified new celltype-specific cis-eQTLs...
2017年发表在Nature Methods杂志上的SCENIC[1]算法,利用单细胞RNA-seq数据,同时进行基因调控网络重建和细胞状态鉴定,应用于肿瘤和小鼠大脑单细胞图谱数据,提出并证明了顺式调控网络分析能够用于指导转录因子和细胞状态的鉴定。SCENIC通过使用生物学驱动的features自动清除肿瘤样本特异性等批次效应。
Figure 1. Schematic of a typical single-cell RNA-seq analysis workflow. 原始数据预处理和比对后获得gene count矩阵后作为本分析流程的开始。 2. 数据预处理 - Pre-processing 数据预处理的目的是获得counts矩阵或者read counts 矩阵,这两者的区别取决于测序时候是否加入UMIs作为分子识别符(molecular identifier)。
最厉害,最全,最新的莫过于Sean Davis小哥在Github上建的awesome-single-cell repository资源库: seandavi/awesome-single-cell 针对于初入门者的single-cell RNA-seq数据分析在线课程资料: Analysis of single…
In recent years, the rapid development of single-cell RNA-sequencing (scRNA-seq) technology has provided the high-resolution and rich data necessary to elucidate disease characteristics and enabled the analysis of complex interactions between individual cells and cell types. The application of scRNA-...
Single-Cell RNA-Seq Analyze transcriptome heterogeneity at the single-cell level. GENEWIZ from Azenta offers optimized workflows for 5’ and 3’ gene expression libraries to uncover cellular differences that are masked by bulk RNA sequencing.