RNA sequencingMULTIOMICSDATA integrationSingle-cell multi-omics data integration aims to reduce the omics difference while keeping the cell type difference. However, it is daunting to model and distinguish the two differences due to cell heterogeneity. Namely, even cells of the same omics and type ...
Here, we comprehensively characterize mouse cardiac progenitor cells (CPCs) marked byandexpression from E7.5 to E9.5 using single-cell RNA sequencing and transposase-accessible chromatin profiling (ATAC-seq). By leveraging on cell-to-cell transcriptome and chromatin accessibility heterogeneity, we ...
Prostate cancer is heterogeneous and patients would benefit from methods that stratify those who are likely to respond to systemic therapy. Here, we employ single-cell assays for transposase-accessible chromatin (ATAC) and RNA sequencing in models of early treatment response and resistance to enzalutam...
RNA + 单细胞表面蛋白(CITE-seq) CITE-seq 大概的实现方法是在 antibody 上连一段 RNA,这样就可以通过测序得到表面蛋白的峰度。 RNA + ATAC: 非常成熟了,10X已经很好的商业化了(虽然可能质量不是太好,远不如之前那篇 SHARE-seq,Cell, 2020),SHARE-seq 的解读可以参考 新技术 | 单细胞多组学 SHARE-seq 及...
https://doi.org/10.1016/j.stem.2020.11.015Get rights and content Under a Creative Commons license Open access Referred to by Evaluation of Ranzoni et al.: Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis Cell Stem Cell, Volume 28, Issue 3, 4 March ...
当然,通量还是比较小。但不可否认的是,现在很多非RNA类Single Cell测序,仍然在采用这样的技术,如Single Cell DNA-Seq,Single Cell ATAC-Seq等,都是采用此技术进行单细胞分选后再分别用不同试剂盒建库。所以可以说,至少在10X和BD,或者其他企业推出有效的Single DNA测序技术之前,C1仍然会是市场上的重要组成部分。
期刊:Cancer Cell (IF: 30.075) 链接:https://doi.org/10.1016/j.ccell.2021.09.010 作者利用单细胞RNA、ATAC和T细胞受体序列测序,构建了TNBC患者肿瘤微环境和外周血来源免疫细胞的高分辨率转录组和表观组动态图谱,系统比较了响应患者和非响应患者的肿瘤微环境及外周血免疫特征,阐明免疫细胞在不同治疗方案下的动态...
Single-cell RNA sequencing (scRNA-seq) allows the comprehensively profiling of the gene expression changes observed in development at a cellular level [19], while single-cell transposase-accessible chromatin sequencing (scATAC-seq) allows the analysis of chromatin accessibility and transcription factor (...
单细胞RNA测序(scRNA-seq)可以在单细胞水平上获取基因表达谱,帮助我们更好地理解生物系统的异质性。单细胞分析已经扩展到其他组学领域,如单细胞ATAC-seq和CITE-seq,分别用于测量染色质的可及性和蛋白质表达。…
Single-cell multi-omics data integration aims to reduce the omics difference while keeping the cell type difference. However, it is daunting to model and distinguish the two differences due to cell heterogeneity. Namely, even cells of the same omics and