Here, we comprehensively characterize mouse cardiac progenitor cells (CPCs) marked byandexpression from E7.5 to E9.5 using single-cell RNA sequencing and transposase-accessible chromatin profiling (ATAC-seq). By leveraging on cell-to-cell transcriptome and chromatin accessibility heterogeneity, we ...
Prostate cancer is heterogeneous and patients would benefit from methods that stratify those who are likely to respond to systemic therapy. Here, we employ single-cell assays for transposase-accessible chromatin (ATAC) and RNA sequencing in models of early treatment response and resistance to enzalutam...
Single-cell RNA sequencing (scRNA-seq) allows the comprehensively profiling of the gene expression changes observed in development at a cellular level [19], while single-cell transposase-accessible chromatin sequencing (scATAC-seq) allows the analysis of chromatin accessibility and transcription factor (...
Single-cell analysis of chromatin silencing programs in development and tumor progression, bioRxiv 2020.09.04.282418 Image 实现的想法也不难,大概对 TN5 做改造,把感兴趣的蛋白(比如 TF )连上去, 这样当蛋白和 DNA 结合时,融合的 TN5 就会切割 DNA,从而实现和ATAC-seq类似的效果。 因此也可以看作是 ATAC-...
To reveal the impact of chromatin accessibility on gene expression, we integrated sNucRNA-seq and sNucATAC-seq data and demonstrated that cell-type-specific marker genes display cell-type-specific patterns of chromatin accessibility. Our data suggest that the differential chromatin accessibility is a ...
期刊:Cancer Cell (IF: 30.075) 链接:https://doi.org/10.1016/j.ccell.2021.09.010 作者利用单细胞RNA、ATAC和T细胞受体序列测序,构建了TNBC患者肿瘤微环境和外周血来源免疫细胞的高分辨率转录组和表观组动态图谱,系统比较了响应患者和非响应患者的肿瘤微环境及外周血免疫特征,阐明免疫细胞在不同治疗方案下的动态...
单细胞还有哪几件嫁衣呢?天凉了,多穿几件吧,RNA+ATAC,RNA+VDJ。 书中概述了单细胞测序和甲基化在疾病和探索的角色,single cell测序和甲基化异质性,尤其是关注单细胞测序和甲基化的方法潜在的应用,对潜在的这些变化与肺疾病之间的相关性,以及导致异质细胞反应的信号通路在治疗中的潜在的角色。
Advances in single-cell RNA sequencing (scRNA-seq) technologies in the past 10 years have had a transformative effect on biomedical research, enabling the profiling and analysis of the transcriptomes of single cells at unprecedented resolution and throug
当然,通量还是比较小。但不可否认的是,现在很多非RNA类Single Cell测序,仍然在采用这样的技术,如Single Cell DNA-Seq,Single Cell ATAC-Seq等,都是采用此技术进行单细胞分选后再分别用不同试剂盒建库。所以可以说,至少在10X和BD,或者其他企业推出有效的Single DNA测序技术之前,C1仍然会是市场上的重要组成部分。
Multimodal models for Integrated Regulatory Analysis, or MIRA, is a python package for analyzing the dynamic processes of gene regulation using single-cell multiomics datasets. MIRA works on top of Scanpy and Anndata to provide a rich, comprehensive framework integrating accessibility and expression dat...