Single-cell T cell and B cell antigen receptor-sequencing data analysis can potentially perform in-depth assessments of adaptive immune cells that inform on understanding immune cell development to tracking clonal expansion in disease and therapy. Howeve
Single-cell immune repertoire sequencing of B and T cells in murine models of infection and autoimmunityAdaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past and current immune responses. Recent advances in single-cell sequencing...
Assessment of single-cell gene expression (single-cell RNA sequencing) and adaptive immune receptor (AIR) sequencing (scVDJ-seq) has been invaluable in studying lymphocyte biology. Here we introduce Dandelion, a computational pipeline for scVDJ-seq analysis. It enables the application of standard V...
Single-cell immune landscape of human atherosclerotic plaques. Nat Med. 2019;25(10):1576–88. Article CAS PubMed PubMed Central Google Scholar Li X, He X, Wang J, Wang D, Cong P, Zhu A, et al. The regulation of exosome-derived miRNA on heterogeneity of macrophages in atherosclerotic ...
The efficacy of neoadjuvant chemo-immunotherapy (NAT) in esophageal squamous cell carcinoma (ESCC) is challenged by the intricate interplay within the tumor microenvironment (TME). Unveiling the immune landscape of ESCC in the context of NAT could shed light on heterogeneity and optimize therapeutic ...
Single-cell immune profiling reveals thymus-seeding populations, T cell commitment, and multilineage development in the human thymus 2022, Science Immunology Azvudine is a thymus-homing anti-SARS-CoV-2 drug effective in treating COVID-19 patients 2021, Signal Transduction and Targeted Therapy Delineat...
cell population, the cells must be categorized on the basis of the expression levels of established surface markers, which might potentially introduce bias. Since human tumors are intricate multicellular ecosystems composed of diverse cells, including subsets of cancer, immune, and stromal cells, their...
Adaptive immunity is based on clonal selection and expansion from a repertoire of T and B lymphocytes bearing a diversity of cell-surface receptors and antibodies that recognize specific antigens. The enormous diversity of lymphocyte receptors and antibodies expressed from a relatively small number of ...
sequencing data, enabling cell type identification at a higher resolution and providing relatively richer gene information. However, the amount of RNA in a single nucleus is still significantly lower than that in whole cells, which suggests that snRNA-seq may not be appropriate for immune cell ...
TCR and BCR repertoire profiling are important to reflect the disease’s adaptive immune status and develop new therapeutics for infectious disease. Therefore, another central part of our study is to assess the repertoire diversity of TCR and BCR and especially their clustering, enabling us to deduc...