High-throughput sequencing of T cell receptor and immunoglobulin loci is used to characterize the breadth of an effector cell response to a stimulus, such as a vaccine or infection. Unique responding effector cell clones and abundance thereof can be determined. Additionally, methods for determining ...
While immune repertoire sequencing datasets have steadily increased from 103to 106sequencing reads per sample [6–10], it has still remained a challenge to extract from large-scale repertoire data immunological status-specific fingerprints ofentirerepertoires for systems medicine and immunodiagnostics applic...
Profile the entire immune repertoire Learn More Single cell Analyze paired chains in individual B and T cells Learn More ADDITIONAL SERVICES Flexible options for your unique project goals Leverage our expertise in immune sequencing and single cell analysis with custom service solutions. Bioinf...
NGS sequencing enables in-depth analysis of the TCR repertoire at an unprecedented level. A greater understanding of the cancer-immunity cycle is now possible, leveraging a deeper understanding of evenness, convergence, and haplotyping to potentially improve patient outcome...
High-throughput sequencing (HTS) of immune repertoires has enabled the quantitative analysis of adaptive immune responses and offers the potential to revolutionize research in lymphocyte biology, vaccine profiling, and monoclonal antibody engineering. Advances in sequencing technology coupled to an exponential...
Preparation of IgG immune repertoire for next generation sequencing The experimental procedures were summarized in Supplementary Fig. S1B. Total RNA was extracted with PAXgene Blood miRNA Kit (QIAGEN). 1.2 μg was reverse-transcribed with an IgG constant region specific primer (Supplementary Table...
Gupta NT, Adams KD, Briggs AW, Timber- lake SC, Vigneault F, Kleinstein SH (2017) Hierarchical clustering can identify B cell clones with high confidence in Ig repertoire sequencing data. J Immunol 198:2489–2499. h t t p s : // d o i . o r g / 1 0 . 4 0 4 9 / j i ...
High-throughput IgH and IgL repertoire sequencing For IgH, a modified version of HTGTS-Rep-Seq48 was used to cover both VDJ and SHM pattern of the whole human VH1-2 sequence. Briefly, starting from 400 ng genomic DNA extracted from sorted splenic GC B cells, a biotinylated human JH2 ...
Eliciting protective titers of HIV-1 broadly neutralizing antibodies (bnAbs) is a goal of HIV-1 vaccine development, but current vaccine strategies have yet to induce bnAbs in humans. Many bnAbs isolated from HIV-1-infected individuals are encoded by imm
However, even with the probably suboptimal help from the weakened CD4+ T cell repertoire, the first CD8+ T cell responses are strong, although their progression into long‑term memory cells could be impaired. The rapid decline of CD8+ T cell responses observed after the founder epitope is ...