RNA-velocity ++ 个人非常喜欢的 RNA velocity,利用 splicing 引入真实的 cell transition 方向。 Image 与之前那些 monocle 等基于表达谱相似来构造trajectory的不同,RNA velocity 是利用转录这一天然的蕴含时间的过程来对 Cell transition 有个刻画。 Image CellRank:思路也很自然结合两者,RNA velocity + 表达谱相似,...
a childhood tumor has been molecularly defined at the primary cancer site, however, the bone marrow (BM) as the metastatic niche of NB is poorly characterized. Here we perform single-cell transcriptomic and epigenomic profiling of BM aspirates from 11 subjects spanning three major NB subtypes and...
we observed a substantial decrease in number of cells with any CellTag reads, a 2.6–2.9-fold reduction in the number of unique CellTags detected, and a loss of CellTag amplicon unique molecular index (UMI) complexity with the 10X Genomics Multiome (RNA + ATAC) kit, as compared to ...
Science封面:Single-cell Genomics 本期《科学》(Science)杂志的封面特刊聚焦单细胞基因组学(Single-cell Genomics),从单细胞组学水平畅谈了人类对免疫系统和脑神经系统的新认识,以及单细胞表观遗传学的新进展,并对“人类细胞图谱”计划寄以厚望。 Single-cell transcriptomics to explore the immune system in health ...
Jun 4, 2023 renv.lock Update the package dependencies in renv.lock Jan 19, 2022 README MIT license redeemR Introduction ReDeeM: single-cellRegulatory multi-omics withDeepMitochondrial mutation profiling. ReDeeM is a single-cell multiomics platform featuring ultra-sensitive mitochondrial DNA (mtDNA) ...
Seurat is an R toolkit for single cell genomics, developed and maintained by the Satija Lab at NYGC. We are excited to release Seurat v5! This updates introduces new functionality for spatial, multimodal, and scalable single-cell analysis. ...
2021年Single Cell Genomics Day在美国东部时间2021年3月26日星期五上午10点至下午5点线上进行。主要议题有: 了解尖端分子技术,包括:单细胞染色质状态和转录因子整合分析,利用单细胞进行体内CRISPR筛选,并进…
BMC Medical Genomics volume 16, Article number: 300 (2023) Cite this article 2490 Accesses 2 Citations Metrics details Abstract Neoadjuvant chemotherapy (NAC) is a well-established treatment modality for locally advanced breast cancer (BC). However, it can also result in severe toxicities while ...
Single-cell RNA sequencing For 3′ scRNAseq, matched HPV16− and HPV16+ rafts generated from isogenic NIKS lines were harvested on day 14 post-plating, dissociated into single cells separately but in parallel, and subjected to 10x Genomics Chromium genomic sequencing. A total of 3833 HPV16−...
Longitudinal single-cell profiling of chemotherapy response in acute myeloid leukemia ArticleOpen access08 March 2023 Inflammatory bone marrow signaling in pediatric acute myeloid leukemia distinguishes patients with poor outcomes ArticleOpen access23 November 2022 ...