RNA-velocity ++ 个人非常喜欢的 RNA velocity,利用 splicing 引入真实的 cell transition 方向。 Image 与之前那些 monocle 等基于表达谱相似来构造trajectory的不同,RNA velocity 是利用转录这一天然的蕴含时间的过程来对 Cell transition 有个刻画。 Image CellRank:思路也很自然结合两者,RNA velocity + 表达谱相似,...
2019年的Single cell genomic day于3月22日在Satija Lab举办,共八位研究者发表了相关研究的演讲。虽然会议的规格不是很高,但是不妨碍他是一场全球单细胞研究者关心的大会。当地时间上午九时Rahul Satija发表了第一篇演讲(PPT如本文所示),算是对这次大会的一个总的概括,他的题目是:Single-cell genomics: Recent ad...
2021年Single Cell Genomics Day在美国东部时间2021年3月26日星期五上午10点至下午5点线上进行。主要议题有: 了解尖端分子技术,包括:单细胞染色质状态和转录因子整合分析,利用单细胞进行体内CRISPR筛选,并进…
干货满满! Single Cell Genomics Day: A Practical Workshop标签: 课程学习 好文要顶 关注我 收藏该文 微信分享 Life·Intelligence 粉丝- 616 关注- 1 +加关注 0 0 « 上一篇: ggplot常见语法汇总查询 » 下一篇: 单细胞数据整合方法 | Comprehensive Integration of Single-Cell Data ...
Science封面:Single-cell Genomics 本期《科学》(Science)杂志的封面特刊聚焦单细胞基因组学(Single-cell Genomics),从单细胞组学水平畅谈了人类对免疫系统和脑神经系统的新认识,以及单细胞表观遗传学的新进展,并对“人类细胞图谱”计划寄以厚望。 Single-cell transcriptomics to explore the immune system in health ...
we use single-cell genomics from natural samples combined with phylogenomics to infer the evolutionary origin of the phylum Picozoa, a globally distributed but seemingly rare group of marine microbial heterotrophic eukaryotes. Strikingly, the analysis of 43 single-cell genomes shows that Picozoa belong...
Single-cell RNA-sequencing library preparation Bone marrow single cells were taken forward for library generation using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics, Inc.) following their recommended protocol. In brief, 10,000 cells were used as input for the Gel Bead...
10XGenomics/cellranger main 1Branch4Tags Code Folders and files Name Last commit message Last commit date Latest commit 10x Genomics feat: Cell Ranger 9.0.0 Dec 11, 2024 fe06169·Dec 11, 2024 History 6 Commits .github Issues and pull requests are not monitored...
cell genomics and epigenomics have facilitated in the context of human health. It highlights the potential of single-cell omics to further advance the development of personalized treatments and precision medicine in cancer, diabetes and chronic age-related diseases. The promise of single-cell ...
cell type (upper panels) and dividing and non-dividing epithelial cells (lower panels) on the indicated days.cGene set enrichment analysis (GSEA) of upregulated genes in club cells on day 2, 3, and 4 compared to day 0 (MAST test, FC > 2,p_adj <0.05). The terms mentioned in ...