We used single-cell and spatial transcriptomics to provide a global view of human embryonic cell-type specification, shedding light on developmental processes such as axial patterning, stage transition, and differences between human and mouse embryonic development....
Fig. 2: Integrated single cell and spatial transcriptomics uncovers different interaction networks in LUAD and LUSC. A Heatmap showing the Pearson correlation between the relative cell-type abundance for each immune cell type (calculated within the CD235− enrichment). Colour indicates the Pearson ...
Recent studies have emphasized the importance of single-cell spatial biology, yet available assays for spatial transcriptomics have limited gene recovery or low spatial resolution. Here we introduce CytoSPACE, an optimization method for mapping individua
Abstract The field of spatial transcriptomics is rapidly expanding, and with it the repertoire of available technologies. However, several of the transcriptome-wide spatial assays do not operate on a single cell level, but rather produce data comprised of contributions from a – potentially heterogeneo...
radial glia neuroepithelial cells single-cell RNA sequencing spatial transcriptomics Introduction During early mammalian development, embryos start from a single-cell zygote and progress to a blastocyst, followed by gastrulation. During this period, embryonic pluripotent stem cells diversify into lineage-spec...
Single-cell RNA sequencing and spatial transcriptomics Single-cell RNA sequencing (scRNA-seq) Bulk RNA sequencing is a traditional sequencing method, which mixes RNA together for sequencing by extracting RNA from cells or tissues. The key distinction between Bulk RNA-seq and scRNA-seq lies in the...
打卡最近的一篇单细胞空间组学的文章,题目是:Embryo-scale, single-cell spatial transcriptomics,更大的scale和单细胞分辨率的方法:sci-Space。 这篇文章的最后的一个作者是Monocle的作者,human fetal gene …
Integration of Single-Cell and Spatial Transcriptomic Data Reveals Spatial Architecture and Potential Biomarkers in Alzheimer's Disease 来自 Springer 喜欢 0 阅读量: 1 作者:X Fan,H Li 摘要: Alzheimer's disease (AD) is a complex neurodegenerative disorder characterized by the gradual loss of neurons...
Single-cell transcriptomics has broadened our understanding of cellular diversity and gene expression dynamics in healthy and diseased tissues. Recently, spatial transcriptomics has emerged as a tool to contextualize single cells in multicellular neighbourhoods and to identify spatially recurrent phenotypes, ...
Cancer-associated fibroblasts (CAFs) are a heterogeneous cell population that plays a crucial role in remodeling the tumor microenvironment (TME). Here, through the integrated analysis of spatial and single-cell transcriptomics data across six common cancer types, we identified four distinct functional ...