analysis pipeline development, computational biology, data analysis tutorial, single‐cell RNA‐seqSingle‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented resolution. The promise of
基于以上问题,作者综述了目前scRNA-seq分析流程、步骤和方法(Figure 1),提出了一套目前最佳的实践分析流程,详见theislab/single-cell-tutorial. 这套分析流程在Jupyter–Ipython notebook进行,包括R和python语言,可推荐作为一套分析模版。 Figure 1. Schematic of a typical single-cell RNA-seq analysis workflow. ...
J. Current best practices in single-cell RNA-seq analysis: a tutorial. Mol. Syst. Biol. 15, e8746 (2019). This scRNA-seq-focused review and workflow template served as inspiration for the current benchmark-based Expert Recommendation. Article PubMed Central PubMed Google Scholar Kharchenko,...
experimental workflow single-cell dissociation, single-cell isolation, library construction, and sequencing 分别为以上四步走 As a first step, asingle‐cell suspension is generatedin a process called single‐cell dissociation in which the tissue is digested. plate-based & droplet-based 但是都存在问题,...
Fig. 1. Schematic overview of a typical single-cell ATAC sequencing analysis workflow. 2. Single-cell ATAC sequencing technologies Within two years of the development of bulk ATAC-seq technology, two different strategies of single-cell adaptations were introduced: split-and-pool combinatorial cellular...
J. Current best practices in single-cell RNA-seq analysis: a tutorial. Mol. Syst. Biol. 15, e8746 (2019). PubMed PubMed Central Google Scholar van der Maaten, L. & Hinton, G. Visualizing data using t-SNE. J. Mach. Learn. Res. 9, 2579–2605 (2008). Google Scholar Blondel, ...
Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis. This is a preview of subscription content...
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Single cell trajectory detection. Contribute to dpeerlab/Palantir development by creating an account on GitHub.
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows you to profile the whole transcriptome of a large number of individual cells. However, the analysis of the large volumes of data generated from these experiments requ