Single-cell sequencing provides analysis of thousands of individual cells within complex environments, as opposed to traditional bulk sequencing methods that only provide a representative average of all cells. With developments in microfluidics and molecular barcoding, NGS-based technologies have increasingly...
基于以上问题,作者综述了目前scRNA-seq分析流程、步骤和方法(Figure 1),提出了一套目前最佳的实践分析流程,详见theislab/single-cell-tutorial. 这套分析流程在Jupyter–Ipython notebook 进行,包括R和python语言,可推荐作为一套分析模版。 Figure 1. Schematic of a typical single-cell RNA-seq analysis workflow. ...
Single-cell RNA sequencing analysis identified 12 B-cell subtypes and characterized the transcriptomic heterogeneity of B cells in muscle-invasive bladder cancer (MIBC). The interferon-stimulated B-cell subtype (B-ISG15) and germinal center-associated B-cell subtypes (B-LMO2, B-STMN1) were signi...
Tagmented genomic DNA undergo nucleic acid amplification and sequencing for generating single-cell whole genome DNA libraries.DHINGRA, DALIARUFF, DAVID
Single-cell sequencing is frequently affected by “omission” due to limitations in sequencing throughput, yet bulk RNA-seq may contain these ostensibly “omitted” cells. Here, we introduce the single cell trajectory blending from Bulk RNA-seq (BulkTraj
Experiment-related workflow of scRNA-seq techniques Advances of individual-cell RNA sequencing in carcinoma Immune cells Non-immune cells Combination of scRNA-seq and other methods Use of individual-cell RNA sequencing inside different fields Conclusions and perspective Author contributions Declaration of in...
Human cancer cell lines have long served as tools for cancer research and drug discovery, but the presence and the source of intra-cell-line heterogeneity remain elusive. Here, we perform single-cell RNA-sequencing and ATAC-sequencing on 42 and 39 human cell lines, respectively, to illustrate ...
Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...
Additionally, the use of TMT multiplexing enables the use of any Orbitrap based mass spectrometer for single-cell proteomics by boosting the signal of the peptides for detection and sequencing by mass spectrometry. Single cell throughput using TMT multiplexing "Thanks to Orbitrap MS and ...
cell RNA sequencing (scRNA-seq) of CD11b+myeloid cells in naïve and GL261 glioma-bearing mice that reveal distinct profiles of microglia, infiltrating monocytes/macrophages and CNS border-associated macrophages. We demonstrate an unforeseen molecular heterogeneity among myeloid cells in naïve and ...