These steps should set up an environment to perform single cell analysis with the tutorial workflow on a Linux system. Please note that we have encountered issues with conda environments on Mac OS. When using Ma
I am following the notebook to better understand the steps of single-cell RNA seq. I am running the notebook on a mac os 10.12.6, the version of R that I am using is 3.6.1. I am running the original tutorial with the data you have kindly provided. I had to change the original ...
Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows you to profile the whole transcriptome of a large number of individual cells. However, the analysis of the large volumes of data generated from these experiments requires specialized statistical and computational ...
analysis pipeline development, computational biology, data analysis tutorial, single‐cell RNA‐seqSingle‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented resolution. The promise of this technology is attracting a growing user base for single‐cell analysis methods. As more...
Current best practices in single-cell RNA-seq analysis: a tutorial 摘要 这篇文章主要阐述了一个典型的单细胞 RNA-seq 分析的详细流程,包括了以下流程: pre-processing -数据预处理 quality control -质量控制 normalization -数据标准化 data correction -数据修正(batch correction, noise correction, etc.) ...
add single cell and bulk tutorial Newer version of the nf-core template is available. Your pipeline is using an old version of the nf-core template: 3.0.2. Please update your pipeline to the latest version. For more documentation on how to update your pipeline, please see thenf-core ...
Welcome to the Loupe V(D)J Browser tutorial. In the next few pages, you will learn how to explore the data generated by a single Cell Ranger run on a human T cell sample spiked in with 40% CMV, 10% Flu, and 5% SARS-CoV-2 (COVID) reactive cells....
Learn how to analyze output data from Cell Ranger, starting withfinding significant features. Dataset description The AML Tutorial dataset contains the results of aCell Ranger aggr pipelineanalysis run for three samples, including two healthy control samples of frozen human bone marrow mononuclear cells...
《Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data》 Introduction 目前基于单细胞测序主要有两种建库技术,一种是主打细胞数量的10X platform,该技术的特点是提出了unique molecular identifiers(UMI),即每一个细胞对应唯一的barcode作为区分于其他细胞的标识符;简而言之就是测到的...
文献阅读-《Tutorial: guidelines for the computational analysis of single-cell RNA sequencing data》 文献名称:教程:单细胞 RNA 测序数据的计算分析指南 文献期刊:nature protocols 发表时间:2021-1-9 摘要 单细胞 RNA 测序技术 (scRNA-seq) 是一种流行且功能强大的技术,可以分析大量单个细胞的整个转录表达谱。