single‐cell RNA‐seqtumor heterogeneityCancers acquire resistance to systemic treatment with platinum‐based chemotherapy (eg, cisplatin [CDDP]) as a result of a dynamic intratumoral heterogeneity (ITH) and clonal repopulation. However, little is known about the influence of chemotherapy on ITH at ...
single‐cell RNA‐seqQuantitative analysis of singleヽell RNA sequencing (RNA﹕eq) is crucial for discovering the heterogeneity of cell populations and understanding the molecular mechanisms in different cells. In this unit we present a bioinformatics workflow for analyzing singleヽell RNA﹕eq data ...
This technique enables researchers to uncover cellular heterogeneity and detect rare cell populations that might be indistinguishable in bulk RNA‐seq data. The primary aim of scRNA‐seq analysis is to investigate cellular heterogeneity and distinguish distinct cell types or states. scRNA‐seq provides ...
Comparison of Microarray and RNA‐seq Analysis Methods for Single Cell Transcriptomics 来自 core.ac.uk 喜欢 0 阅读量: 26 作者: FB Yu 摘要: Behavior of single cells can be explained through changes in the transcription level of the genome followed by translation of the resulting mRNA into ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
Inference and analysis of cell-cell communication of non-myeloid circulating cells in late sepsis based on single-cell RNA-seqYanyan Tao, Miaomiao Li, Cheng Liu First published: 22 November 2024 https://doi.org/10.1049/syb2.12109The authors wish it to be known that, in their opinion, Ya...
Single-cell sampling with RNA-seq analysis plays an important role in reference laboratory; cytogenomic diagnosis for specimens on glass-slides or rare cells in circulating blood for tumor and geneti...
In addition, we performed single cell RNA-seq analysis on SIN3A knockdown embryos. Differential expression analysis between SIN3A knockdown and control morula embryos (Figure 4G-H) identified 459 genes disrupted by SIN3A knockdown (adjusted P < .05, Kruskal-Wallis test). These genes are ...
•To avoid repetition, under “Experimental validation,”“standard approaches” refers to the following: immunofluorescence (IF) and immunohistochemical (IHC) staining; RNA in situ hybridization or RNAscope; and bulk RNaseq of sorted cells, scRNA-seq.•Given space limitation, we restricted ...
Single-cell RNA sequencing (scRNA-seq) is ideally suited to dissect tumor cell heterogeneity and its microenvironment as shown for MM11 and cell types in the BME of MM patients9. However, an integrated scRNA-seq analysis of both tumor cells and BME from the same patient sample, as well as...