2.2 shRNA-resistant Gankyrin表达产物的Western Blot鉴定 经Western Blot检测,抵抗型质粒re-Gankyrin转入HEK293T细胞后正确表达,在26 000 Da附近特异蛋白质条带增强,与Gankyrin蛋白质分子量相符。 图3 shRNA-resistant Gankyrin载体双酶切鉴定结果1—shRNA-resistant Gankyrin载体未经酶切2—shRNA-resistant Gankyrin ...
本研究利用PCR介导的定点突变技术,构建了shRNA—resistantGankyrin抵抗型质粒。经酶切鉴定及DNA测序证实序列完全正确,抵抗型质粒构建成功;经WesternBlot检测,抵抗型质粒在HEK293T细胞中正确表达,为进一步研究Gankyrin在乳腺癌转移中的作用及其分子机制提供了重要的实验材料。赵青解放军总医院柏兆方解放军302医院徐广解放军总...
本研究利用PCR介导的定点突变技术,构建了shRNA—resistantGankyrin抵抗型质粒。经酶切鉴定及DNA测序证实序列完全正确,抵抗型质粒构建成功;经WesternBlot检测,抵抗型质粒在HEK293T细胞中正确表达,为进一步研究Gankyrin在乳腺癌转移中的作用及其分子机制提供了重要的实验材料。
gankyrin是近年来发现的一种癌基因,研究表明其表达产物Gankyrin与肿瘤发生密切相关.实验室前期工作发现Gankyrin表达水平与临床乳腺癌转移存在正相关,并可能通过多个环节影响乳腺癌的转移.本研究利用PCR介导的定点突变技术,构建了shRNA-resistant Gankyrin抵抗型质粒.经酶切鉴定及DNA测序证实序列完全正确,抵抗型质粒构建成功;经...
本研究利用PCR介导的定点突变技术,构建了shRNA.resistantGanl~yrin抵抗型质粒。经酶切鉴定及DNA测序证实序列完全正确,抵抗型质粒构建成功;经WesternBlot检测.抵抗型质粒在HEK293T细胞中正确表达,为进一步研究Gankyrin在乳腺癌转移中的作用及其分子机制提供了重要的实验材料。关键词Gankyrin定点突变抵抗型质粒RNA干扰回复中...
phenotype.Surprisingly,the MDR phenotype was completely reversed in two transected clones.Conclusions This study demonstrates that MDR can be reversed by the shRNA-mediated RNAi in K562/ADM cells,which provides a valuable clue as to sensitizing multidrug-resistant hepatoma cells to anti-cancer drugs....
GC5基因型的E.coli 菌株(endA, recA, T1/T5 phage resistant) 100ul的菌液 Mission™ shRNA Plasmid DNA 可直接用于转染的质粒 1ug,20ul的规格 可用于瞬时或稳定的shRNA转染――载体含有puromycin筛选标记 与相应的包装质粒共转染包装细胞(HEK 293T)包装出病毒颗粒 ...
Mildew resistant plant BC 1 -2 also had a 1-kb H. bulbosum DNA fragment identified with a ten-base random primer using polymerase chain reaction (PCR... J Xu,KJ Kasha - 《Theoretical & Applied Genetics》 被引量: 116发表: 1992年 Overcoming Two Post-fertilization Genetic Barriers in Intersp...
In order to inhibit AS expression, we established a permanent AS gene knockdown cell line via transduction of lentiviruses carrying shRNA against AS mRNA. The cell model we chose was MCF-7 which was resistant to rADI treatment with high amount of endogenous AS expression. At the first, ...
Objective To explore the inhibitory effect of shRNA expression vector for mdr1 gene on the expression of P-gp in amycin-resistant human erythroleukemia cell strain K562/ADM. 目的探讨靶向mdr1基因的shRNA表达载体对耐阿霉素的人红白血病细胞(K562/ADM)P-gp表达的抑制作用。 更多例句>> 3...