Gene therapy, Recombinant adeno-associated virus, Autosomal-dominant, Retinal degen-eration, Allele-independent, Short-hairpin RNA, shRNA-resistant cDNARecombinant adeno-associated virus (rAAV) has become an important gene delivery vector for the treatment of inherited retinal degenerative diseases. Many...
Rh123）effluxassay.ResultsThestabletransfectedclonesshowedvariousdegreesofreversalofMDRphenotype.Surprisingly,theMDRphenotypewascompletelyreversedintwotransectedclones.ConclusionsThisstudydemonstratesthatMDRcanbereversedbytheshRNA-mediatedRNAiinK562/ADMcells,whichprovidesavaluableclueastosensitizingmultidrug-resistanthepatoma...
This cassette allows L193 to be used as a Gateway Destination Vector and requires that the vector is propagated in a ccdB-resistant strain. All plasmid constructs were verified by DNA sequencing. Production of retroviruses, transduction and induction of shRNA expression Retroviral destination vectors ...
中国协和医科大学博士学位论文靶向VEGF和VEGFR2的shRNA在抗肿瘤研究中的应用姓名:***学位级别:博士专业:微生物与生化药学指导教师:**光20060501中国医学科学院博士论文中文摘要获得稳定系细胞后,首先考察其在增殖方面的变化。结果显示HTl080一V3细胞与对照组细胞相比,生长速度和克隆形成率都未改变,说明肿瘤细胞在体外的增...
片段类型:ORF;cDNA;shRNA;sgRNA;ncRNA;miRNA;cirRNA等 片段物种:空载体 原核抗性:Amp 产品特点 L202 pPLVPTH2-tTR-KRAB-Cerulean-scrambled-shRNA载体序列是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长...
Objective To explore the inhibitory effect ofshRNA expression vectorfor mdr1 gene on the expression of P-gp in amycin-resistant human erythroleukemia cell strain K562/ADM. 目的探讨靶向mdr1基因的shRNA表达载体对耐阿霉素的人红白血病细胞(K562/ADM)P-gp表达的抑制作用。
2. Objective To explore the inhibitory effect of shRNA expression vector for mdr1 gene on the expression of P-gp in amycin-resistant human erythroleukemia cell strain K562/ADM. 目的探讨靶向mdr1基因的shRNA表达载体对耐阿霉素的人红白血病细胞(K562/ADM)P-gp表达的抑制作用。
Elevated RPL35A expression was found in glioma cells relative to normal astrocytes29, while RPL35A overexpression in Jurkat cells (an acute T-cell leukemia derived cell line) produced a cell death resistant phenotype30. The resistance of Jurkat cells overexpressing RPL35A was not accompanied by ...
The only reports on miR-636 describe miR-636 as a potential candidate responsible for the post-transcriptional silencing of GR in GC-resistant cells.35 For the first time, our study suggests that miR-636 is a tumor suppressor miRNA affecting HCC tumorigenesis via suppression of the Ras ...
However, the incidence of sponta neous colitis in IL-10−/-mice on a colitis resistant C57BL/ 6J inbred background is low and prolonged. Here, we use piroxicam to accelerate the course of IL-10-deficient mice, International Journal of Nanomedicine 2021:16 Powered by TCPDF (www.tcpdf...