GC5基因型的E.coli 菌株(endA, recA, T1/T5 phage resistant) 100ul的菌液 2周到货 Mission™ shRNA Plasmid DNA 可直接用于转染的质粒 1ug,20ul的规格 可用于瞬时或稳定的shRNA转染――载体含有puromycin筛选标记 与相应的包装质粒共转染包装细胞(HEK 293T)包装出病毒颗粒 3周到货 Mission...
Rescue of shRNA–knockdown with shRNA–resistant dcDNA.Hong, HaoDouglas, S. KimBernward, KlockeKory, R. JohnsonKairong, CuiNorimoto, GotohChongzhi, ZangJanina, GregorskiLinn, GieserWeiqun, Peng
本研究利用PCR介导的定点突变技术,构建了shRNA.resistantGanl~yrin抵抗型质粒。经酶切鉴定及DNA测序证实序列完全正确,抵抗型质粒构建成功;经WesternBlot检测.抵抗型质粒在HEK293T细胞中正确表达,为进一步研究Gankyrin在乳腺癌转移中的作用及其分子机制提供了重要的实验材料。关键词Gankyrin定点突变抵抗型质粒RNA干扰回复中...
Recombinational rescue of the stalled DNA replication fork: a model based on analysis of an Escherichia coli strain with a chromosome region difficult to r... To examine the physiological effects of DNA replication arrest at the terminus (Ter), we constructed a replication-blocked Escherichia coli...
因此,RNA干扰介导的knockdown实验需要rescue对照来说明,前期的实验结果不是由于Off-target效应而产生的,即knockdown之后做resistant质粒的过表达,看是否有细胞表型的逆转,这也是为了说明knockdown的特异性。 基因定点突变是指按照设计的要求,使基因的特定序列发生插入、删除、置换和重排等变异,是研究蛋白质结构和功能之间的...
In vivo activity of nuclease-resistant siRNAs RNA, 10 (2004), pp. 766-771 Google Scholar [208] K. Kariko, M. Buckstein, H. Ni, D. Weissman Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA Immunity, 23 (2005)...
Partial rescue of shRNA-mediated loss of CAR shedding using an shRNA-resistant mutant of ADAM10.Houri, NadiaHuang, KuoChengNalbantoglu, Josephine