Short-read whole genome sequencing for determina- tion of antimicrobial resistance mechanisms and capsular serotypes of current invasive Streptococcus agalactiae recovered in the USA. Clin Microbiol Infect. 2017;23(8):574.e7-574.e14Metcalf, B.J., et al., Short-read whole genome sequencing for ...
The debate between short-read and long-read sequencing is an ongoing one, but it is clear that both technologies have their own unique benefits. For the most comprehensive results, it is best to combine the two together. By doing so, you can get the most complete picture of your data whi...
Short-read whole-genome sequencing data are often applied to large-scale bacterial comparative genomics projects but the reconstruction of plasmids from these data is facing severe limitations, such as the inability to distinguish plasmids from each other in a bacterial genome. We developed gplas, a...
Short-read Illumina whole-genome sequencing (GS; 30× coverage) was performed on DNA isolated from the cell lines. Sequencing was performed by Macrogen (South Korea) on Illumina HiSeq X machines with Illumina TruSeq PCR-free chemistry. After quality control (QC), reads were aligned to the GR...
shotgun sequencing of whole genomic DNA using short-read data and no available closely related reference genome (e.g. from the same species or genus)... H Mcpherson,MVD Merwe,SK Delaney,... - 《Bmc Ecology》 被引量: 147发表: 2013年 Copy number variant detection in inbred strains from...
The Onso system is an innovative benchtop short-read sequencing platform that uses PacBio SBB technology to produce an unprecedented level of accuracy. Learn more Talk with an expert If you have a question, need to check the status of an order, or are interested in purchasing an instrument, ...
In recent years, many software packages for identifying structural variants (SVs) using whole-genome sequencing data have been released. When published, a new method is commonly compared with those already available, but this tends to be selective and in
The method had been proven highly suitable for single nucleotide polymorphism analysis and sequencing of short stretches of DNA. Pyrosequencing had been successful for both confirmatory sequencing and de novo sequencing. By increasing the read length to higher scores and by shortening the sequence ...
Validated whole exome sequencing (WES) using third-party hybrid capture protocols Single-cell 3’ single-cell RNA-Seq libraries supported and validated on the Onso systemBlog Why SBB could be a game-changer for liquid biopsy research In this article we will investigate how an important new...
Whole genome sequencing Short read sequencing libraries were prepared with an Illumina Nextera XT DNA Library Preparation Kit and sequenced on an Illumina MiSeq instrument with a 250-bp paired-end protocol (MiSeq v3 chemistry) according to the manufacturer’s instructions. Trimming of the short re...