This chapter includes a list of selected therapeutic targets, possessing SH 2 or SH 3 domains. The design of specific antagonists to these domains holds the promise of targeted treatment of a broad range of pathologies. The role of SH 2 and SH 3 domains in signal transduction has been ...
The amphiphysins are brain-enriched proteins, implicated in clathrin-mediated endocytosis, that interact with dynamin through their SH3 domains. To elucidate the nature of this interaction, we have solved the crystal structure of the amphiphysin-2 (Amph2) SH3 domain to 2.2 . The structure possess...
GRB2, a small protein comprising one SH2 domain and two SH3 domains, represents the human homologue of the Caenorhabditis elegans protein, sem-5. Both GRB2 and sem-5 have been implicated in a highly conserved mechanism that regulates p21ras signalling by receptor tyrosine kinases. In this rep...
However, it is now evident that factors other than somatic mutations also play an important role in MPN disease initiation and progression, among which germline predisposition (single-nucleotide polymorphisms and haplotypes) may strongly influence the occurrence of MPNs. In this context, the LNK ...
These data indicate that tyrosine phosphorylation of two different phosphoproteins may play a role during src transformation either by directing their interaction with pp60src, by redirecting subcellular distribution or both. 展开 关键词: PHOSPHOTYROSINE PROTEIN COMPLEXES SRC HOMOLOGY DOMAIN TRANSFORMATION ...
Background: SH2 domains have a fundamental role in signal transduction, These domains interact with proteins containing phosphorylated tyrosine residues and, in doing so, mediate the interactions of proteins involved in tyrosine kinase signalling. The issue of specificity in SH2 domain interactions is ...
SH2 domains are widespread protein‐binding modules that recognize phosphotyrosines and play central roles in intracellular signalling pathways. The SH2 domain of the human protein tyrosine kinase Fyn has been expressed, purified and crystallized in the unbound state and in complex with a high‐affini...
The Src homology 2 (SH2) domain is a modular protein interaction domain that specifically recognizes the phosphotyrosine (pY) motif of a target molecule. We recently reported that a large majority of human SH2 domains tightly bind membrane lipids, and many show high lipid specificity. Most of ...
We expressed c‐Src in yeast to test the role of the SH2 and SH3 domains of Src in the negative regulation exerted by Tyr527 phosphorylation. Inducible expression of c‐Src in Schizosaccharomyces pombe caused cell death. Co‐expression of Csk counteracted this effect. Src proteins mutated in...
利用RT-PCR技术将人源性PLC-γ1中的SH2-SH2-SH3结构域基因扩增并克隆到载体pGEX-2T中,经热激反应转化到大肠杆菌BL21菌株内,在低温(33℃)下、利用IPTG诱导表达构建的重组基因的高效表达体系,利用谷胱甘肽琼脂糖(sepharose)4B纯化得到高表达的重组蛋白。SDS-PAGE和Western blot检测表明,PLC-γ1的SH2-SH2-SH3结...