Note: The appropriate flask size depends on the number of cells frozen in the cryovial, and the culture environment varies based on the cell and media type. If the cell density is too low and the cells have been in culture for 4-5 days, we recommend concentrating the cells by centrifugi...
Note: The appropriate flask size depends on the number of cells frozen in the cryovial, and the culture environment varies based on the cell and media type. We typically recommend dislodging Sf9 cells mechanically rather than enzymatically (i.e., with trypsin/EDTA). To do so, wrap your fl...
1 Sf9 细胞的悬浮培养 在25 cm2T-Flask培养瓶 Conning 中贴壁培养的Sf9细胞将其传入100 ml摇瓶 培养液体积为30 ml 在27℃、 100 rpm下悬浮培养并用血细胞计数板确定其密度 活细胞由0.4%(w/v)台盼蓝染色法排除 摇瓶的初始细胞浓度是5~6×105 cells.ml-1。 培养基成分为 Grace’s 昆虫培养基中加入 10...
The transferability of this system to bioreactor scale was also successfully achieved, attaining a 1.4‐fold increase in VLP production and maintaining a higher cell viability than shake flask controls. Analysis of the metabolism of stable cell pools and parental Sf9 cells did not show significant ...
rAAV was produced on the basis of this cell culture process; the maximum cell density reached 22.8 × 106 cells/mL, and the viral vector titre was 16 × 1011 VG/mL. On the basis of this shake flask process, small-scale and pilot-scale reactor processes were developed. In ...
Sf9 cells were grown to maximal concentration, 8 x 10(6) cells/ml in a 500ml spinner flask, with a doubling time at the exponentially growing phase of 24.5 hours, using serum-free media. To explore the ability of Sf9 cells to be infected by the Japanese encephalitis (JE) virus Beijing...
Normally, the volume of a suspension culture in shaker flasks should not exceed 40% of the flask volume. This ensures optimal mixing of the medium and oxygenation. Sf9 cell suspension cultures should be passaged three times weekly and seeded at a density of ~ 1 × 106 cells/mL. It is ...
Continuous insect cell (Sf-9) culture with aeration through sparging The continuous growth of Spodoptera frugiperda Sf-9 cells in a 250-ml blown-glass jacketed spinner flask under a direct air sparging environment was inves... MY Wang,WE Bentley - 《Applied Microbiology & Biotechnology》 被引量...
One hour post-infection cells were transferred into a spinner flask, and fresh medium was added to reach a final cell density of 106 cells/ml. Cells were grown for an additional 72 h at 22°C with constant stirring at 80 rpm and collected by centrifugation. After extensive washing with ...
This paper describes the use of a new membrane-aerated spinner flask which was compared to conventional surface-aerated spinner flasks with regard to growth of the insect cell line Sf9 and recombinant protein production after infection with baculovirus. Using a commercially available serum-free ...