Some of them are based on the label‐free phase holographic microscopy. In this technique, the low‐power (635 nm) red diode laser divided into two beams—reference and an object beam—that passes through the unlabeled cell cultures on T‐flask surface merged together can be recorded as th...
†The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used. Back to the Gibco Cell Culture Basics homepage Relevant product categories Gibco media Cell dissociation reagents Balanced salt solutions ...
Acteoside: 33.31 mg L−1 in shake flask and 120.13 mg L−1 in bioreactor 2022 [40] In addition to flask culture, there have been many reports using bioreactor or hairy root cultures to produce valuable PhGs. For example, an internal loop airlift bioreactor with a sifter riser was ...
View Cell Culture Bottle Flask Plates XY Stage Inverted Fluorescent Microscope Product Description Same Objective for Both Bright Field & Phase Contrast ObservationNot Necessary to Change Different Objective Same Objective for Both Bright Field & Phase Contrast Observatio...
(0.22 μm) nitrogen gas and enclosing in a capped culture flask (working volume: 20 mL), cells were incubated in static conditions under continuous light illumination (~150 μmol photons m−2s−1) at 28 °C for 2 days prior to cell sorting. For the evaluation of the ...
Expansion of A-NK cells Add the A-NK cell culture medium containing 1 × 106/ml of irradiated feeder cells to the flasks with selected A-NK cells. The final volume of the medium including feeder cells should be the same as that of A-NK cell inducing medium (e.g. 10 ml/T75 flask)...
In brief, a single colony was transferred to 20 mL 2xTY medium supplemented with AMP at a final concentration of 100 µg/mL in a 100 mL conical flask and grown over night (O/N) (16 h) at 37 °C, 220 RPM. The following day, the O/N culture was transferred to a ...
The obtained strain BL7992 successfully synthesized 1.13 mg/L melatonin by utilizing L-tryptophan (l-Trp) as a substrate in a shake flask. It was determined that the rate-limiting enzyme for melatonin synthesis is the arylalkylamine N-acetyltransferase, which is encoded by the HsAANAT gene. ...
The composite cell count requirement into cell suspension culture flask, the culture bottle is placed in the box 5%CO2 and 37 DEG C 2-4 hours (or 24-48 hours) after the change of liquid culture to change liquid, time by cell circumstances. ...
For TMTA66 strains, the highest FPase activity was approximately 1.3-fold greater than that of the parental strain RUT C30 at 120 h of cultivation in a shake flask using avicel as the sole carbon source. The FPase activity (U/mg biomass) in TMTA139 strains was approximately 26.5-fold ...