Using pH adapted Sf9 cells, we are presently determining optimal transfection and expression conditions using design of experiment (DOE). However, lower titers are anticipated in comparison to the Baculovirus system. As a result, productivity of transiently transfected Sf9 cells will be further ...
Transfection of Sf9 Insect Cells DNA and siRNA Transfection Short Protocol - DNA Short Protocol - siRNA DNA/siRNA Co-transfection siRNA Transfection Reagent GenMute PepMute PepMute™ Plus LipoJet™ 技術 pH dependent conformational changes (PDCC)修飾的生物可降解聚合物 Simulated virus cell penetratin...
Optimized for suspension Invitrogen FreeStyle 293-F cells Cellfectin II Transfection Reagent Optimal transfection of insect cells, including S2, Sf9, Sf21, and Invitrogen High Five cells DMRIE-C Transfection Reagent Transfection of suspension cells, including CHO, lymphoid, and Jurkat cell lines Exp...
Optimized for suspension Invitrogen FreeStyle 293-F cells Cellfectin II Transfection Reagent Optimal transfection of insect cells, including S2, Sf9, Sf21, and Invitrogen High Five cells DMRIE-C Transfection Reagent Transfection of suspension cells, including CHO, lymphoid, and Jurkat cell lines...
Usage of Sinofection in different cell lines(96-well plate) Cell type Culture medium Cells per well DNA Sinofection Medium change after 4-6h 293H DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS 293FT DMEM 3×104 0.2µg 0.5µL DMEM + 10%FBS 293E DMEM 3×104 0.2µg 0....
Optimized for suspension Invitrogen FreeStyle 293-F cells Cellfectin II Transfection Reagent Optimal transfection of insect cells, including S2, Sf9, Sf21, and Invitrogen High Five cells DMRIE-C Transfection Reagent Transfection of suspension cells, including CHO, lymphoid, and Jurkat cell lines Exp...
产品编号: 3081750 规格: Lipid reagent for transient and stable transfection of mammalian and insect cells. 包装规格: 1 ML 产品类别: 仪器耗材 品牌: Sigma-Aldrich 优惠价: 立即咨询产品价格 产品编号 包装单位 单价(元) 国内现货 国外库存 询价单 3081750 1 ML 3900Escort TM IV 转染试剂 EscortTM IV ...
•particlebombardment,delivernucleicacidtoculturedcellsaswellastocellsinvivo(微小的金颗粒,硅纳米颗粒)ViralMethods •analternativemethodfordeliveryofforeigngenesintocellsandasapossibleinvivooption•Adenoviralvectors•herpessimplexvirus•Retroviruses,Lentiviruses(e.g.,HIV-1)areofparticularinterestbecausetheyhave...
of other types of reagents. GenJet™ was shown to deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines. GenJet™ reagent...
5. Gene expression is tested after incubation with 5% CO2 at 37℃ for 48-72h(incubate sf9 cell line at 27℃ for 48-72h). Fig. 1 Adherent cells' Transfection Attention: 1). The experiment conditions for different cell lines are shown in Table 1. The corresponding transfection protocols...