The invention relates to the field of biological gene engineering, and provides a sequencing method for increasing sequencing reading length. The sequencing method provided by the invention comprises the following steps of: firstly, combining a first anchor primer on a first connector of a nucleic ...
Isoform sequencing (Iso-Seq) uses long-read technology to produce highly accurate full-length reads of mRNA transcripts. Visualization of individual mRNA molecules can reveal new details of transcript variation within understudied portions of mRNA, such as the 5′ untranslated region (UTR). ...
With the development of long-read RNA sequencing, it is now possible to sequence cDNA transcripts in their entirety—without assembly. These full-length sequences improve genome annotation and provide a way to look at gene expression data in an isoform- or allele-specific way. Here, we highlig...
Full-length cDNA (FLcDNA) sequencing establishes the precise primary structure of individual gene transcripts. From two libraries representing 27 B73 tissues and abiotic stress treatments, 27,455 high-quality FLcDNAs were sequenced. The average transcript length was 1.44 kb including 218 bases and 321...
Nearly full-length genome sequencing of HIV-1 using peripheral blood mononuclear cells (PBMC) DNA as a template for PCR is now a relatively routine laboratory procedure. However, this has not been the case when using virion RNA as the template and this has made full genome analysis of circula...
We introduce blend-seq, a method for combining data from traditional short-read sequencing pipelines with low-coverage long reads, with the goal of substantially improving variant discovery for single samples without the full cost of high-co...
Full-length tran sequencing provides insight into lignan and lignin metabolism relationFlaxIso-SeqIlluminalignanligninmetabolismFlax ( Linum usitatissimum L.) is an important economic crop worldwide. The lignin content of flax stems directly determines the quality of flax fiber, and excessively high ...
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be
297) studied the Variable Length Sequencing Problem (VLSP), showed it is NP-complete, presented a polynomial time algorithm for a very restricted version and an approximation algorithm for a slightly less restricted version. In this paper, we pin-point the difficulty by showing that it is NP-...
The use of SMARTer PCR c DNA Synthesis Kit and the Iso Seq data analysis pipeline was recommended to facilitate full-length transcriptome sequencing. However, the transcriptome data quality could be affected by ribosomal RNA contamination, cros...