之后再进行 SMRT 建库和 Pacbio 测序。由于 CCS/subreads 中分子长度提高了大约5倍,因此称之为5x 全长转录组测序技术。)这种方法大大提高了 Pacbio sequel II 有效转录本 full-length 产出(提升了大约8倍),使得一个sequel II flow cell 测2000多个细...
The present disclosure provides a method for analyzing a polyribonucleotide, wherein the polyribonucleotide is an m RNA molecule with a poly(A) tail, said method comprising: (a) obtaining a labeled polyribonucleotide by GI tailing; followed by (b) providing a second molecule comprising a first...
Full-length tran sequencing provides insight into lignan and lignin metabolism relationFlaxIso-SeqIlluminalignanligninmetabolismFlax ( Linum usitatissimum L.) is an important economic crop worldwide. The lignin content of flax stems directly determines the quality of flax fiber, and excessively high ...
full-length mRNAs from MCF-7 human breast cancer cells were sequenced on 147 SMRT cells using the Iso-Seq method on the Pacific Biosciences RSII platform (Additional file1: Table S1). Before sequencing, parts of the sequencing library were size selected to allow for a good representation of ...
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be
The Next Generation Sequencing(NGS) technology, particularly the Illumina platform now has produced most of the animal and plant transcriptomes, but the short reads from NGS sequencers result in incompletely assembled transcripts which are lack ...
Second-generation sequencing technology TGS: Third-generation sequencing DEG: Differentially expressed gene TF: Transcription factor ROIs: Reads of insertion CCS: Circular consensus FLNC: Full-Length non-chimeric Read ICE: Iterative isoform-clustering ...
High-throughput sequencing of the full-length 16S rRNA gene has improved the taxonomic classification of prokaryotes found in natural environments. However, sequencing of shorter regions from the same gene, like the V4-V5 region, can provide more cost-effective high throughput. It is unclear which...
3.1. High-quality reads obtained from the single molecule sequencing-derived transcriptome To obtain a representative full-length transcriptome for C. sativus, total RNAs extracted from the entire plant at full-bloom stage were sequenced on two SMRT cells using the PacBio Sequel system. This generat...
Matsuo, Y., Komiya, S., Yasumizu, Y. et al. Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution. BMC Microbiol 21, 35 (2021)。 PacBio 16S全长测序:一种高效且经济的微生物组研究方法。