These sequencing-based technologies are increasingly being targeted to individual cells, which will allow many new and longstanding questions to be addressed. For example, single-cell genomics will help to uncover cell lineage relationships; single-cell transcriptomics will supplant the coarse notion of ...
More recently, single-cell sequencing technologies have uncovered previously unappreciated levels of cancer and immune cell heterogeneity. These technologies have helped define molecular profiles associated with immune cell activation and exhaustion. However, because this type of analysis demands that the tiss...
SEQUENCING-BASED PROTEOMICS 专利内容由知识产权出版社提供 专利名称:SEQUENCING-BASED PROTEOMICS 发明人:ZHANG, Feng,SCHMID-BURGK, Jonathan Leo,HORNUNG, Veit 申请号:US2019/0294 88 申请日:201904 26 公开号:WO2019/210268A2 公开日:20191031 专利附图: 摘要:The invention provides a cell library for use ...
High-throughput sequencing-based genome-wide identification of microRNAs expressed in developing cotton seeds. Sci China Life Sci, 2015, 58: 778-786Wang YM, Ding Y, Yu DW, Xue W, Liu JY. High-throughput sequencing- based genome-wide identification of microRNAs expressed in developing cotton ...
DNA-based sequencing-based typingClass IIClass IRefinement of SBT strategies leading to the current methodsAmbiguous allele combinationsPCR and sequencing primer design problemsAllele drop outMethods still too slow, complicated and expensiveHLA-DPBHLA-DPAHLA-DRBHLA-DQBHLA-DQAHLA-CHLA-BHLA-AFuture ...
Sequencing-based methods for detection and quantitation of ribose methylations in RNA. Ribose methylation is one of the most abundant RNA modifications and is found in all domains of life and all major classes of RNA (rRNA, tRNA, and mRNA). R... Nicolai,Krogh,Henrik,... - 《Methods》 被...
The breast cancer can be sporadic or familial. Studies in the early 1990s lead to the recognition of the role of BRCA1 and BRCA2 genes in hereditary breast cancer. Next generation sequencing (NGS) technologies have permitted expansion of the number of ge
qRT-PCR analysis of nine selected genes fromP. xylostellawhich showed differential expression after parasitization based on deep sequencing analysis. Error bars indicate standard deviations of averages from three replicates. Fold changes are shown in brackets. ...
Future integrated single-cell analyses based on high-throughput sequencing will enable the simultaneous analysis of genomic, transcriptomic and epigenomic states of cells. Such data will reveal the ancestries of cells, their types, their current functional states and may be used to infer the types ...
Finally, we offer an outlook for another type of technology that leads to pathogen identification using the sequence data obtained in sequencing-based virus hunting. 展开 关键词: infectious molecular clone next-generation sequencer nucleic acid extraction pathogen identification ...