sequencing-based typingClass IIClass IRefinement of SBT strategies leading to the current methodsAmbiguous allele combinationsPCR and sequencing primer design problemsAllele drop outMethods still too slow, complicated and expensiveHLA-DPBHLA-DPAHLA-DRBHLA-DQBHLA-DQAHLA-CHLA-BHLA-AFuture role and ...
Sequencing based typing reveals new insights in DPA polymorphismAn HLA-DPA1 sequencing-based typing (SBT) system has been developed to identify DPA1 alleles. Up to now eight DPA1 alleles have been defined. Six can be discriminated based upon exon 2 polymorphism. The three subtypes of DPA1*01...
Rozemuller EH, Bouwens AG, van Oort E, Versluis LF, Marsh SG, Bodmer JG, Tilanus MG (1995) Sequencing-based typing reveals new insight in HLA-DPA1 polymorphism. Tissue Antigens 45:57–62Rozemuller EH, Bouwens AG, van Oort E, Versluis LF, Marsh SGE, Bodmer JG, Tilanus MG (1995) ...
Characterization of the novel HLA‐DRB1*01:100 allele by sequencing‐based typingHLAHLA‐DRB1*01:100novel allelesequencing‐based typingstatement HLA〥RB1*01:100 differs from HLA〥RB1*01:01:01 by one nucleotide substitution in codon 228 in exon 5. This article is protected by copyright. All ...
HLA‐A*11:324novel allelesequencing‐based typingHLA-A*11:324 differs from HLA-A*11:01:01:01 by one nucleotide substitution in codon 317 in exon 6. This article is protected by copyright. All rights reserved.doi:10.1111/tan.13561Marine Cargou...
Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele. The HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B ...
typing, PCRusing sequence-specific primers (PCR-SSP) whileothers can usedmore efficiently allelelevel resol-ution, PCRusing sequence-specific oligonucleotides (PCR- SSO) probesused twomethods have updatedcontinuously increasingallelic diversity High-resolutiontyping based directsequencing polymorphic...
PCR-SSO typing indicated the presence of possible new HLA-DPB1 variants in the Ethiopian population. The use of the SBT technique, which considers polymorphic as well as constant regions in the second exon of HLA genes, allowed direct identification of two new allelic variants. Moreover,......
Multilocus sequence typing (MLST) revealed that they belonged to 72 sequence types (STs), including 52 new STs. A total of 334 resistance genes of 30 antibiotic resistance genotypes were identified from the genomes, more than half (55.99%, 187/334) of which were 尾-lactamase ge...
A Sequencing Based Typing method (S.B.T.) was first developed for DPB and DRB genes. We report here an S.B.T. method for DQA.The DQA alleles are amplified with two primers, which are chosen in the generic regions of exon 2. The 5′ primer has the -21M13 recognition sequence, ...