The enzyme yield and purification achieved by ATP process is significantly higher than from a comparative precipitation process.doi:10.1016/S0149-6395(00)80055-0Rohit HarveRakesh BajpaiSeparation Science & TechnologyRohit Harve,Rakesh Bajpai.Separation of nucleic acids and proteins. Separation Science and...
Journal of Liquid ChromatographyAgris P.F., Armstrong S.A., Guenther R.H., 1989, “Bio-Macromolecular Liquid Chromatography Yesterday and Today: Advances in the Separation and Study of Nucleic Acids and their Related Proteins”, J. Liquid Chromatogr. 12(8), 1347–1366....
Rapid, repeatable, and resolved chromatographic separations of molecules and analyses of their components have finally arrived in the commercial marketplace. This arrival has been long awaited, particularly with regard to nucleic acids (DNA and RNA). Biotechnological advances of protein and nucleic acid...
This unit describes the use of a column made of a unique anion‐exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The procedure employs ... K Budelier,J Schorr - Curr Protoc Mol Biol 被引量:...
Tumor cells resistant to chloroethylnitrosourea (CENU) therapy contain high levels of O6-alkylguanine DNA-alkyltransferase (GATase), a DNA repair enzyme th... Gonzaga Prescilla E,Brent Thomas P - 《Nucleic Acids Research》 被引量: 71发表: 1989年 Purification and characterization of human and ...
Aqueous two-phase systems are useful tools for fractionation and studies of cell components including proteins and nucleic acids. The systems consist of two non-miscible liquid layers which both have high content of water. The purification achieved by partition of biosubstances between the two liquid...
Separation apprattus and process for fabricating separation appratus A separation apparatus wherein a large number of wells are formed in the inner wall of a channel, through which a sample passes, to form a sample separation region. In case of separating nucleic acids and proteins, the maximum...
The term hydrophilic-interaction chromatography is proposed for this variant of normal-phase chromatography. This mode of chromatography is of general utility. Mixtures of proteins, peptides, amino acids, oligonucleotides, and carbohydrates are all resolved, with selectivity complementary to those of ...
MNPs also affect liquid structures and thus are incorporated in the procedures of aqueous extraction and purification, to create a functionally active surface to capture biomacromolecules like nucleic acids, bacteria and proteins [12,115]. Since metals constitute the vast portion of MNPs, iron oxides...
Bioreactive molecules can be absorbed or coupled to their surface, and used to separate biological materials (cells, proteins, nucleic acids, etc.) Dynabeads magnetic beads come in several sizes and different surface functionalities for use in a wide variety of applications. Some beads are precoupl...