The knowledge about the assignment of protein and nucleic acid bands is the key to understand the underlying molecular variations. Tables are compiled for bands of protein secondary structures, bands of nucleotides, and bands of nucleic acids conformations. Figures present Raman spectra of (i) ...
and Gronenborn, A M.: Determining the structures of large proteins and protein complexes by NMR. Trends Biotechnol. 16, 22-34, 1998. :... GM Clore,AM Gronenborn - Springer US 被引量: 304发表: 2002年 Recommendations for the presentation of NMR structures of proteins and nucleic acids. ...
Isotopically labeled methyl groups provide NMR probes in large, otherwise deuterated proteins. However, the resonance assignment constitutes a bottleneck for broader applicability of methyl-based NMR. Here, we present the automated MethylFLYA method for
Protein secondary structure assignment, a subdiscipline of computational chemistry, is yet to be explored using deep learning techniques. Protein secondary structure elements are assigned to support structural analysis and prediction. Algorithms like DSSP, generally regarded as the gold standard for ...
Universal Structure Alignment of Monomeric and Complex Structure of Nucleic Acids and Proteins - pylelab/USalign
30S ribosome stability and integrity of NMR samples High resolution NMR experiments require that the sample of the macromolecular complex has to be pure from contaminating proteins and nucleic acids, it must be monodispersed and due to the experimental restraints of the technique highly concentrated. ...
Nucleic Acids Res. 47, D330–D338 (2019). Zhou, N. et al. The CAFA challenge reports improved protein function prediction and new functional annotations for hundreds of genes through experimental screens. Genome Biol. 20, 244 (2019). Article Google Scholar LeCun, Y., Bengio, Y. & ...
D. Interactions between p53, hMSH2-hMSH6 and HMGI(Y) on Holliday junctions and bulged bases. Nucleic Acids Res. 30, 2427–2434 (2002). Article CAS PubMed PubMed Central Google Scholar Reeves, R. & Adair, J. E. Role of high mobility group (HMG) chromatin proteins in DNA repair. ...
A novel methodology is described for the assignment of disulfide bonds in proteins of known sequence. The denatured protein is subjected to limited reduction by tris(2-carboxyethyl)phosphine (TCEP) in pH 3.0 citrate buffer to produce a mixture of partially reduced protein isomers; the nascent sulfh...
Differentially abundant proteins (0 g/GC) were grouped based on their GO biological process and molecular function tags obtained automatically with DAVID and manual assignment from UniProt. Proteins with increased and decreased abundance were grouped separately. In the increased abundance grouping, 275 pr...