采用半巢式聚合酶链反应(semi-nested pCR)对日本献血员、各种肝病患者及静脉毒瘾者等高危人群血清进行检测,结果在献 …www.fx120.net|基于9个网页 2. 半巢式聚合酶链式反应 ...460位点进行单链构象多态性分析(SNP),同时以半巢式聚合酶链式反应(Semi-nested PCR)对IL-4-588位点进行多态性分析.结…new.med....
and amplifying for 35 cycles on the second PCR reaction system mixed liquid by using the first PCR reaction product as a template, wherein the final PCR product is used for agarose gel electrophoresis; and adding the PCR product into the enzyme digestion reaction liquid, and carrying out agaros...
gondii oocysts in soils in northwest China using a novel semi-nested PCR assay, which provided baseline data for the effective prevention and control of toxoplasmosis in this region.doi:10.1186/s12917-014-0238-zWang, MengMeng, PengYe, Qiang...
seminested PCRStandard PCR was ineffective in detecting a baculovirus-derived chitinase transgene in the T1 generation of chitinase-expressing Nicotiana tabacum cv. CF80 after leaves were flue-cured at high temperatures. Consequently, a seminested PCR method was developed using fresh leaves from T2 ...
Highly sensitive seminested RT-PCR systems for the specific detection of genotype I and II small round structured viruses (SRSVs) were developed based on the nucleic acid information deposited in the databanks. SRSVs could be detected in 107-fold dilutions of three different stool samples. In ...
Three rounds of semi-nested PCR on cM and cCV DNA have allowed the identification of circulating foetal DNA in maternal plasma. This technique may be utilised for non-invasive prenatal diagnosis of specific β-globin gene mutations from foetal DNA in maternal plasma. 展开 ...
Using a cloned HCMV DNA as a template, we found that the one-tube semi-nested PCR-ELISA gave a strong positive response when 20 copies of the template were used, whereas both the hybridization-based and the semi-nested-based PCR-ELISA required at least 200 template copies. The claim of ...
A rapid, sensitive and specific semi-nested RT-PCR was developed to detect and differentiate virulent and avirulent strains of Newcastle disease virus (NDV). For a total of 67 NDV strains, the results obtained from the semi-nested RT-PCR were consistent with those from nucleotide sequence analy...
Bacteriological isolation and identification of Mycoplasma species is difficult and time-consuming, therefore, molecular identification of Mycoplasma using PCR targeting specific genes is considered a specific and sensitive method for identification. The aim of current study was to isolate, characterize Mycopl...
Development of a semi-nested PCR for the improved detection of Mycoplasma bovis from bovine milk and mucosal samples. Vet. Microbiol. 91: 91-100.Hayman B,Hirst R.Development of a semi-nested PCR for the improved detection of Mycoplasma bovis from bovine milk and mucosal samples.Veterinary ...