We developed a semi-nested PCR protocol for fungal 18S ribosomal RNA genes coupled to subsequent analysis of the PCR products by Temperature Gradient Gel Electrophoresis (TGGE) to monitor the fungal community structure in aquatic habitats characterized by a pollution gradient. Our TGGE-protocol was ...
The semi-nested RT-PCR protocol was used to detect CDV RNA in 9 of the 10 cases that were positive by at least 1 of the immunologic (FA and IHC) methods. Sequence data indicated that the amplified strains of CDV are more closely related to a naturally occurring strain than to a ...
Highly sensitive seminested RT-PCR systems for the specific detection of genotype I and II small round structured viruses (SRSVs) were developed based on the nucleic acid information deposited in the databanks. SRSVs could be detected in 107-fold dilutions of three different stool samples. In ...
As an application of the PCR detection protocol, environmental water samples were screened for the presence of V. cholerae and Shigella spp. Positive amplifications resulted from V. cholerae and Shigella species in environmental samples. The results obtained indicate that the described seminested PCR ...
The real- time PCR protocol began with one cycle at 95°C for 5 min followed by 50 cycles of: 95°C for 15 s, 60°C for 10 s and 72°C for 20 s. Immediately after amplification, a re-annealing cycle consisting of 95°C for 1 min and a rapid cooling to 70°C for 1 min ...
Pfmdr1 codons 86, 184 and 1246 and Pfcrt codon 76 were genotyped by PCR–RFLP and sequencing. Results Among 1129 included children, the prevalence of plasmodial infection was 79.5 % at Lastourville, 53.6 % at Fougamou, 36.1 % at Koula-Moutou, and 21.2 % at Franceville. The prevalence ...
(Supports LIN protocol versions 1.3, 2.0, 2.1, 2.2A, and SAE J2602)Low Power SPI (LPSPI) 1x 2x 2x 3xLow Power I2C (LPI2C) 1x 1x 2x1x 2x 3x 3x 3xFlexCAN(CAN-FD ISO/CD 11898-1) (1x with FD) (1x with FD) (1x with FD) (2x with FD) (3x with FD)...
Res. Public Health 2024, 21, 636 4 of 26 was selected, and the workshop protocol was further developed by the facilitator (MW, MPhil Cantab, independent consultant/creative researcher and facilitator, woman) with researcher input (LB and RT). Two YPAG members (AR, RM) also contributed to...
Kconfig files describe build-time configuration options (called symbols in Kconfig-speak), how they’re grouped into menus and sub-menus, and dependencies between them that determine what configurations are valid.Kconfig files appear throughout the directory tree. For example, subsys/power/Kconfig def...
PCRLateral flowPlasmodium falciparumBackground A rapid, non-invasive, and inexpensive point-of-care (POC) diagnostic for malaria followed by therapeutic intervention would improve the ability to control infection in endemic areas. Methods A semi-nested PCR amplification protocol is described for ...