A multiplex RT-PCR that simultaneously identifies 12 respiratory viruses including HRV is also routinely used in our lab. Here we have described the specific steps of methods for identification of HRV from clinical samples, such as sample preparation, isolation of total RNA, nested-RT-PCR for ...
摘要: Although standard reverse transcriptase-polymerase chain reaction (RT-PCR) is a remarkably sensitive technique, its sensitivity can be further increased by performing "nested" RT-PCR. This involves...DOI: 10.1385/1-59259-283-X:065
Ninty-five cervicovaginal samples were randomly selected; each was tested for HPV DNA and genotypes using 3 methods in parallel: Multiplex-PCR, the Nested PCR followed by Sanger sequencing, and the Next_Gen Sequencing (NGS) with two assays (NGS-A1, NGS-A2). The study was approved by the ...
The results were found to be comparable with a total of 60% (36/60) of samples being positive for HPV using the single-tube assay, while 62% (37/60) positivity was found with two-tube PCR assay. We succeeded in developing a single-tube seminested PCR method for HPV DNA detection ...
A nested PCR has been successfully developed to detect rabies virus. It could detect a volume of 3 TCID 50 of rabies virus and gave a positive result of 0.8 pg of RNA. The positive results were only from rabies virus strains CVS, HEP flury, RC HL, ERA, 1088, Komatsugawa by two pairs...
Thus, this QN-RT-PCR method is more sensitive than the real-time PCR. When the same samples were examined by real-time PCR and our QN-RT-PCR method, in one patient in clinical remission after chemotherapy and allogeneic-bone madoi:10.1111/j.1365-2257.2004.00587.xM....
1.4 病毒效价的测定 照常规方法进行,按Reed和Munch法计算TCID50。1.5 RNA提取 用Isogen(日本产)法提取狂犬病病毒的RNA,按说明书操作,经紫外分光光度计测定D260和D280值,计算所提取RNA的量和纯度。1.6 cDNA的合成和NestedPCR的扩增 君,已阅读到文档的结尾了呢~~...
Highly sensitive seminested RT-PCR systems for the specific detection of genotype I and II small round structured viruses (SRSVs) were developed based on the nucleic acid information deposited in the databanks. SRSVs could be detected in 107-fold dilutions of three different stool samples. In ...
A rapid, sensitive and specific semi-nested RT-PCR was developed to detect and differentiate virulent and avirulent strains of Newcastle disease virus (NDV). For a total of 67 NDV strains, the results obtained from the semi-nested RT-PCR were consistent with those from nucleotide sequence analy...
Molecular methods used, as Semi Nested Multiplex Malaria PCR (SnM-PCR) for the correct species diagnosis Rubio et al 1999a, Rubio et al 1999b and Genotyping PCR for the detect polymorphism of the merozoite surface proteins 1 and 2 (MSP-1 and MSP-2) to characterize the different populations...