Selectable markergenes are a vital part of most transformation protocols. They are delivered alongside the gene of interest, either on the same plasmid or on a separate plasmid. A wide range of selectable marker regimes is available and is particularly important in species where transformation effici...
The present invention provides a precursor plasmid for preparing a plasmid without selectable markers, including: 1) a replication original site; 2) a selectable marker gene; 3) a target gene or a cloning site for inserting the target gene; and 4) paired recombination sites. The paired ...
1a). Both enabled successful selection when both N- and C-markertrons were transduced yielding >95% BFP + mCherry + double transgenic cells in selected cultures compared to <40% double-positive cells in non-selected culture (Fig. 2b; Plasmid pairs 3, 4 and 5, 6). Cells ...
What would happen if a plasmid without a selectable marker was chosen ... 01:56 How does over exploitation of beneficial species affect biodiversity ?... 03:15 Draw a well labelled diagram to show various stages of fertilization. 03:53 Draw the well labelled diagram of a mature human sperm...
Thus, we were unable to develop a functional puromycin selectable marker in A. baumannii despite the functional activity displayed by E. coli transformants. This difficulty could be due to the use of genetic elements that have not been optimized for expression in A. baumannii such as the promot...
词典 例句 词条selectable marker在英语 » 德语中的译文 (跳至德语 » 英语) selectable marker名词 selectablemarker selektierbarerMarker 您希望如何使用 PONS.com? 广告版的PONS 像往常一样访问PONS.com并进行广告跟踪和广告投放 接受并继续有关追踪的详细信息,请参阅数据保护信息和隐私设置。
The ability to stack multiple genes in plants is of great importance in the development of crops with desirable traits but can be challenging due to limited selectable marker options. Here we establish split selectable marker systems using protein splicing elements called “inteins” for Agrobacterium...
In order to implement a genetic manipulation for site-directed HR of exogenous genes into the BmT1 site ofP. pastoris, a plasmid vector was constructed using the toxic genemazFas the counter-selectable marker under the control of PAOX1ofP. pastoris. A failure scenario happened that the DNA ele...
The resulting plasmid with the cloned fragment in the correct orientation is named pGBAMD-11 and is the replacement vector that is used to delete the A. niger amdS gene using the MARKER GENE FREE™ approach. Example 3.2 Inactivation of the Endogenous A. niger amdS Gene Prior to ...
Eyl 105 was transformed with a plasmid containing the selectable marker hygromycin phosp... K Tang,A Wu,J Yao,... - 《Acta Biotechnologica》 被引量: 19发表: 2000年 A Seed Treatment for Eliminating Non-hybrid Plants when using Atrazine Resistance as a Genetic Marker for Hybrid Seed ...