The study underscores the importance of early detection through Whole Genome Sequencing to identify potential emergent virulence, resistance/ virulence plasmids, and new antimicrobial resistance markers. This approach provides actionable public health data, supporting the development of rob...
in the production of GM foods or for therapeutic purposes. A rise in antimicrobial resistance is the biggest danger, according to recent research. Due to a lack of information on alternate methods, ARMGs are still employed in the production of GM crops today. The creation of GM crops without...
Since it has been established that the injection of plasmid DNA can lead to an efficient expression of a specific protein in vivo, nonviral gene therapy approaches have been considerably improved, allowing clinical trials. However, the use of antibiotic resistance genes as selection markers for plas...
42,43,44,45 Otherwise, the plasmids must undergo an additional step to remove the resistance marker.24,37,46 We made several innovations toward a streamlined, generalizable ARG-free cloning and maintenance strategy. We engineered plasmids with dual auxotrophic and essential gene markers and developed...
Occurrence and Transfer of Plasmids for Antibiotic Resistance and Enterotoxin Production in Enterotoxigenic Escherichia coli of Swine Origin 来自 Wiley 喜欢 0 阅读量: 10 作者:G.,Semjén,L.,Pesti 摘要: SummaryMost of the porcine enterotoxigenic and drug resistant E. coli isolated in Hungary carry a...
The aim of this study was to investigate the possibility that sublethal food preservation stresses (high/low temperature, osmotic and pH stress) can alter the rate of horizontal transmission of antibiotic resistance (ABR) plasmids between Escherichia coli strains and between E. coli and Salmonella se...
The role of broad-host range IncP-1ε plasmids in the dissemination of antibiotic resistance in agricultural systems has not yet been investigated. These plasmids were detected in total DNA from all of 16 manure samples and in arable soil based on a novel 5′-nuclease assay for real-time PCR...
it is possible to predict the resistance phenotype and adapt a specific treatment. The strategies for identifying resistance markers are very similar to those aiming at identifyingvirulence genes(Didelot et al., 2012). In France, comparative genomics of a multidrug resistant and a wild-typeAcinetobac...
We investigated whether persisters can also promote the spread of resistance plasmids. In contrast to mutations, the transfer of resistance plasmids requires the co-occurrence of both a donor and a recipient bacterial strain. For our experiments, we chose the facultative intracellular entero-pathogen ...
Click to launch & play an online audio visual presentation by Prof. Hatch Stokes on Plasmids, integrons and the spread of antibiotic resistance, part of a collection of multimedia lectures.