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Nuclear pore complexes (NPCs) are very large proteinaceous assemblies that consist of more than 500 individual proteins1,2. NPCs are essential for nucleocytoplasmic transport of different cellular components, and disruption of the integrity of NPCs has b
In addition, we show that in plants K63-Ub chains are involved in selective autophagy through NBR1, the second major pathway delivering cargoes to the vacuole for degradation. Similar to autophagy-defective mutants, ubc35-1 ubc36-1 plants display an accumulation of autophagy markers. Moreover, ...
Selective autophagy is a catabolic process with which cellular material is specifically targeted for degradation by lysosomes. The function of selective autophagic degradation of self-components in the regulation of innate immunity is still unclear. Here
Proteins were co-immunoprecipitated with GFP beads out of cells grown to log phase. (f) Quantification of (e). The amount of Gyp1-HA bound to GFP-Atg8 was set to 100%. Immunoblots were probed with HA (top) or GFP antibodies (bottom). Molecular mass markers are in kDa. Asterisks or...
Worms were cultured and main- tained on nematode growth medium (NGM) plates with Escherichia coli OP50-1 in 3 cm or 6 cm dishes30. Plasmids used in this study. pGH8 was a gift from Erik Jorgensen (Addgene plasmid # 19359; https ://n2t.net/addgene:19359; ...
Similar modulation of other mitotic markers such as cyclin B levels has also been shown in response to MPS1 inhibition (Colombo et al, 2010; Tardif et al, 2011). Although direct inhibition of MPS1 autophosphorylation using phospho-specific antibodies has been reported in cells (Sliedrecht et ...
When grown in nonfermentable glycerol medium, cells contain mitochondria that are active in respiration. In those cells, we visualized mitochondria and vacuoles using green fluorescent protein (GFP) and red fluorescent protein (RFP) markers (mito-GFP and vac-RFP), respectively (Figure 1A). We ...
The shuttle cloning vector can be employed to amplify DNA sequences in prokaryotic systems. The shuttle cloning vector may be prepared from plasmids generally used in prokaryotic systems and in particular in bacteria. Thus, for example, the shuttle cloning vector may be derived from plasmids such ...
Killer plasmids contain the coding region of human p53 genes under control of a constitutive CMV promoter/enhancer. FIGS. 3A-3C show trigger evaluation with a reporter construct. Relative β-galactosidase activity in cells transfected with UASG -βGal reporter and other constructs are presented. (...