Vaccine development targeting rapidly evolving pathogens such as HIV-1 requires induction of broadly neutralizing antibodies (bnAbs) with conserved paratopes and mutations, and in some cases, the same Ig-heavy chains. The current trial-and-error search f
The selectins are a family of integral membrane proteins characterized by the presence of lectin-, epidermal growth fac- tor, and complement-binding domains (25, 26). L-selectin was previously known as LEC.CAM-1, LECAM-I, LAM I, and as the MEL-14, Leu-8, TQ1, and Dreg-56 antigen...
Three strains of Levilactobacillus brevis with syntenous glutamate decarboxylase (GAD) operons were evaluated for overall growth, glutamate utilization, and GABA production in typical synthetic growth media supplemented with monosodium glutamate (MSG). Levilactobacillus brevis Lbr-6108™ (Lbr-6108), form...
Methylmercury (MeHg) is a microbially produced neurotoxin derived from inorganic mercury (Hg), which accumulation in rice represents a major health concern to humans. However, the microbial control of MeHg dynamics in the environment remains elusive. Here, leveraging three rice paddy fields with disti...
This makes evaluation of the drug effect difficult. Theragnostic biomarkers (that is, biomarkers that detect and monitor biochemical effects of the drug) may help solve some of these problems. Here, we review three pathological processes that are thought to be involved in the complex surge of AD...
The pipeline has been developed and streamlined by comparing its predictions to manually curated gene models in three fungal genomes and validated against the high-quality gene annotation of Neurospora crassa; SnowyOwl predicted N. crassa genes with 83% sensitivity and 65% specificity. SnowyOwl gains ...
yeast two-hybrid systems are the typical tools for such screening of candidate proteinsin vivo24,25,26,27. Among them, split-ubiquitin system is a well-established, useful technique to screen the candidate proteins with the PPIs for membrane target proteins28,29. As in other yeast systems, sm...
These tech- niques are required for screening of large numbers of proteins and are preferable in the in vivo cellular context. In particular, yeast two-hybrid systems are the typical tools for such screening of candidate proteins in vivo24–27. Among them, split-ubiquitin system is a well-...